姜黄素对B-NHL细胞系Raji细胞转录共激活因子P300表达的抑制作用  

Inhibitory efectory of curcumin on transcriptional co-activator P300 expression of B-NHL cell line Raji cell

作　　者：吴青[1] 陈燕[1] 李新刚[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院血液病研究所,湖北武汉430022

出　　处：《中国药理学通报》2005年第10期1180-1183,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No30271672)

摘　　要：目的探讨姜黄素对B细胞淋巴瘤细胞系Raji细胞转录共激活因子P300的影响及对Raji细胞的作用及其机制。方法以不同浓度的姜黄素作用于体外培养Raji细胞,MTT法检测细胞生长抑制率,应用RT-PCR和蛋白印迹(W estern b lot)法检测Raji细胞中P300的表达。结果①姜黄素具有明显的抑制Raji细胞生长作用,并呈明显的量效关系。②姜黄素作用24 h后,随着浓度剂量的增加P300的mRNA和蛋白表达而逐渐降低。低剂量(6.25μmol.L-1)组P300表达有一定的降低,但与对照组相比差异无显著性(P>0.05),而中、高剂量(12.5、25及50μmol.L-1)则抑制P300的表达(P<0.05)。结论姜黄素对B细胞淋巴瘤细胞系Raji细胞具有抗肿瘤细胞的增殖作用,并呈浓度依赖性。姜黄素能够抑制转录共激活因子P300的表达,可能是其重要的机制。To investigate the effect of curcumin relating transcriptional co-activator P300 on B-NHL cell line Raji, and to study anti-proliferative molecular machenism of curcumin on Raji cells. Methods Raji cells in cultured in vitro were treated by different concentrations of curcumin. The inhibitory ratio of the cells was measured by MTT assay. The expressions of P300 on Raji cells were checked by RT-PCR and Western blot. Results ①Curcumin could inhibit the growth of Raji cells and suppression was both in time-and dosedependent manner.②Curcumin treated with Raji cells for 24 h,with the rise of concentration, can down-rogulate the expressions of both P300 mRNA and protein greatly. There were no significant difference of expres-sions of P300 between control and low-exposed cultures （ 6.25μmol·L^-1 , P 〉 0. 05 ） , but the expression of P300 decreased at intermedieate and high concentration （ 12. 5,25,50μmol·L^-1, P 〈 0. 05 ）. Conclusion Curcumin is able to inhibit the proliferation of Raji cells and dose-dependent manner, the inhibition of the Transcriptional co-activator P300 expression may be the most important mechanism.

关 键 词：姜黄素 转录共激活因子P300 Raji CELL 

分 类 号：R282.71[医药卫生—中药学] R284.1[医药卫生—中医学]