雌二醇和孕酮对人成骨细胞胰岛素受体底物基因表达的时间与剂量依赖性  

Effects of dose-and time-dependence of estradiol and progesterone on the gene expression of insulin receptor substrate in human osteoblasts

作　　者：黄秋霞[1] 周后德[1] 杜巍[1] 何玉玲[1] 廖二元[1] 

机构地区：[1]中南大学湘雅二医院代谢内分泌研究所

出　　处：《中华老年医学杂志》2005年第10期775-778,共4页Chinese Journal of Geriatrics

基　　金：国家自然科学基金资助项目(30400218)

摘　　要：目的观察雌二醇和孕酮对人成骨细胞和人成骨肉瘤MG63细胞胰岛素受体底物(IRS)mRNA表达的影响。探讨雌、孕激素参与骨代谢的作用机制。方法用半定量RTPCR检测人成骨细胞和人成骨肉瘤细胞IRS1、IRS2mRNA的表达量。结果雌二醇或孕酮均诱导MG63细胞IRS2mRNA的表达上调,具有剂量依赖性(P<0.05)和时效依赖性(P<0.05)。其中分别以10-8mol/L的雌二醇干预24h和10-8mol/L的孕酮干预12h作用最明显,分别为对照组的(421±68)%和(327±54)%(均P<0.01)。雌二醇或孕酮共同干预进一步促进MG63细胞和人成骨细胞IRS2mRNA表达的上调,分别为对照组的(496±54)%和(452±58)%(均为P<0.01)。雌二醇和孕酮不影响人成骨细胞和MG63细胞IRS1mRNA的表达(P>0.05)。结论雌二醇或孕酮均可使人成骨细胞和MG63细胞的IRS2mRNA表达上调,且存在剂量与时间依赖性;但不影响IRS1mRNA的表达。Objective To characterize the effects of estradiol（E2 ） and progesterone （P） on the gene expression of IRS families in cultured normal human osteoblast-like cells （HOB） and human osteosarcoma cell line MG-63 in order to understand the mechanism of E2and P acting on the bone. Methods Semi-quantitative RT-PCR was used to study the action of E2 and P on the expression of mRNA of IRS family. Results 10^-10-10^-6 mol/I, of E2 and/or P could up-regulate the gene expression of IRS2 mRNA in a dose- and time-dependent manners in MG-63 cells（P〈0.05）, the maximal effect to stimulate the expression of IRS-2 was in 10^-8mol/L at 24 h for E2 （421±68）%（P〈0.01）, and 10 ^-8 M at 12h for P（327±54）% （P〈0.01）. Combined intervention of E2 and P in MG-63 cells and HOB could further increase the expression of IRS-2 mRNA to（496 × 54）% （P〈0.01） and to（452±58）%（P〈0.01）versus the control, respectively. E and Phad no effects on the expression of IRS-1 mRNA in MG-63 cells or HOB. Conclusions E2 and P can dose and time dependently up-regulate the expression of IRS-2 mRNA in MG-63 cells and HOB. Combined intervention of E2 and P can positively create a synergistic effect on the up-regulation of IRS-2 mRNA. But E2 and P have no effects on the expression of IRS-1 mRNA in MG-63 cells or HOB.

关 键 词：成骨细胞 受体 胰岛素 基因表达 雌二醇 孕酮 

分 类 号：R68[医药卫生—骨科学]