原位杂交检测pal-1mRNA在秀丽小杆线虫野生型和突变体早期胚胎中的分布  

Distribution of pal-1 mRNA in Caenorhabditis elegans wild-type and mutant early (embryos) via in situ hybridization

作　　者：杨玉荣[1] 傅兴[1] 

机构地区：[1]厦门大学生命科学学院,厦门361005

出　　处：《动物学报》2005年第5期884-891,共8页ACTA ZOOLOGICA SINICA

基　　金：国家自然科学基金(No.30370695);教育部留学回国人员启动基金;厦门大学校级基金资助~~

摘　　要：pal-1是秀丽小杆线虫(Caenorhabditis elegans)早期胚胎发育中决定体细胞命运的重要基因,也是转录因子,调控后续基因的表达,凡含有该基因表达的细胞发育成体细胞。本文通过整体原位杂交技术检测pal-1mRNA在C·elegans野生型和par-1、par-2、par-3、par-4突变体、spn-4突变体、mex-5/mex-6突变体早期胚胎中的分布,探讨这些基因在胚胎发育早期对pal-1mRNA的影响。实验结果表明:par-1、par-3、par-4突变使4细胞胚胎pal-1mRNA完全丧失了野生型不对称分布模式,pal-1mRNA分布在所有卵裂球中;par-2对pal-1mRNA的分布影响较小,在par-2突变体4细胞胚胎中pal-1mRNA分布与野生型相同。spn-4、mex-5、mex-6也能影响pal-1mRNA的分布,使其分布丧失不对称性。在par-1、par-4突变的情况下,pal-1mRNA广泛存在,但PAL-1蛋白也不表达,显示对pal-1mRNA的翻译调控是PAL-1蛋白空间和时序不对称分布的主要原因[动物学报51(5):884-891,2005]。This paper reports the distribution of pal-1 mRNA in Caenorhabditis elegans early embryo of wild type, par- 1 , par-2, par-3, par-4 mutant, spn-4 mutant and rnex-5/mex-6 mutant via in situ hybridization. In wild type embryos, pal-1 mRNA showed asymmetric localization in one cell, two cell and 4-cell embryos. At 4-cell embryo, posterior 2 cells showed higher levels than anterior cells. In par-1 , par-3, par-4 mutant embryos, pal-1mRNA lost asymmetric localization and were present everywhere. Loss function of par-2 did not affect the distribution of pal-1mRNA, spn-4, mex-5 and mex-6 also affected pal-1mRNA asymmetric distribution. In par-1, par-4 mutant, pal-1mRNA was present everywhere but no PAL-1 was detected in any cells of 4-cell embryos. This implies that the translational regulation of pal-1mRNA may be the reason for spalially and temporally asymmetric location of PAL-1 protein [ Acta Zoologica Sinica 51 （5）： 884-891, 2005].

关 键 词：秀丽小杆线虫 PAR PAL-1 spn-4 mex-5 mex-6原位杂交 

分 类 号：Q78[生物学—分子生物学]