猪卵巢体外保存温度对卵母细胞染色质构型和成熟能力的影响  

Effects of ovary storage temperature on chromatin configuration and in vitro maturation of porcine oocytes

作　　者：岳奎忠[1] 陈新[2] 孙兴参[1] 李子义[1] 朱赫[1] 卞志明[1] 谭景和[3] 

机构地区：[1]东北农业大学生命科学学院,哈尔滨150030 [2]解放军空军总医院,北京100000 [3]山东农业大学动物科技学院,山东泰安271018

出　　处：《动物学报》2005年第5期919-923,共5页ACTA ZOOLOGICA SINICA

基　　金：国家自然科学基金(No.30170679);国家科技部"973"项目(No.G200016108)资助~~

摘　　要：Most porcine oocytes used in studies on embryo biotechnology and the in vitro production of embryos are currently obtained from the ovaries of slaughtered gilts. The duration and temperature during ovary transportation and handling might, therefore, affect the recovery of culturable COCs, chromatin configuration and developmental competence of oocytes. The effects of ovary storage temperature on chromatin configuration and in vitro maturation of porcine oocytes were examined in this study. Ovaries collected from a slaughterhouse were stored in vitro for 8 h under different temperatures. The results showed that more culturable COCs were isolated from the ovares stored at 39℃ than that from ovaries stored at 31℃ or 20℃ and before storage. Thirty-one centidegree was the best storage temperature in terms of cumulus expansion, nuclear maturation and morphology of the first polar body after in vitro maturation culture. The ability of cumulus expansion was completely lost in COCs derived from ovaries stored at 39℃for 8 hours. Ovary storage (at both 31℃ and at 20℃) increased the proportion of oocytes with the GVc configuration in which chromatin condensed into a single big clump at the nucleolus and the functional significance of this configuration needs further investigations.Most porcine oocytes used in studies on embryo biotechnology and the in vitro production of embryos are currently obtained from the ovaries of slaughtered gilts. The duration and temperature during ovary transportation and handling might, therefore, affect the recovery of culturable COCs, chromatin configuration and developmental competence of oocytes. The effects of ovary storage temperature on chromatin configuration and in vitro maturation of porcine oocytes were examined in this study. Ovaries collected from a slaughterhouse were stored in vitro for 8 h under different temperatures. The results showed that more culturable COCs were isolated from the ovares stored at 39℃ than that from ovaries stored at 31℃ or 20℃ and before storage. Thirty-one centidegree was the best storage temperature in terms of cumulus expansion, nuclear maturation and morphology of the first polar body after in vitro maturation culture. The ability of cumulus expansion was completely lost in COCs derived from ovaries stored at 39℃ for 8 hours. Ovary storage （at both 31℃ and at 20℃ ） increased the proportion of oocytes with the GVc configuration in which chromatin condensed into a single big clump at the nucleolus and the functional significance of this configuration needs further investigations [ Acta Zoologica Sinica 51 （5）： 919 923, 2005].

关 键 词：猪 卵巢 体外保存 保存温度 卵母细胞 染色质构型 成熟能力 

分 类 号：S828[农业科学—畜牧学]