缺血预处理对家兔肢体缺血再灌注后肺抗氧化系统的影响  

Effect of ischemic preconditioning of limb on anti-oxidative system in the development of lung injury associated with limb ischemia-reperfusion in rabbits

作　　者：陈雷[1] 金可可[2] 潘景业[1] 金广健[1] 王健[1] 许益笑[2] 

机构地区：[1]温州医学院附属第一医院骨科,浙江温州325000 [2]温州医学院病理生理教研室

出　　处：《中国骨伤》2005年第10期607-609,共3页China Journal of Orthopaedics and Traumatology

基　　金：浙江省中医药科研基金项目(No.2004c124)

摘　　要：目的观察肢体缺血预处理对家兔肢体缺血再灌注后肺损伤时肺抗氧化系统的影响并探讨其机制。方法健康家兔30只,随机分为假手术对照组(SC)、缺血再灌注组(IR)和缺血预处理组(IPC),每组10只。实验结束时,取肺组织测定超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)、黄嘌呤氧化酶(XOD)活性和丙二醛(MDA)、一氧化氮(NO)含量,同时计算肺系数及进行肺病理学检查。结果肢体缺血再灌注后肺组织SOD、GSH-PX活性和NO含量显著下降,XOD活性和MDA含量明显增高,肺系数升高,肺组织病变明显,肢体缺血预处理可明显缓解上述变化。结论家兔肢体缺血再灌注后,肺抗氧化功能下降参与肺脏的损伤过程,肢体缺血预处理对这种肺损伤有明显的保护作用。Objective：To investigate the effect of ischemic preconditioning on anti-oxidative system in the development of lung injury associated with limb ischemia-reperfusion in rabbits. Methods：30 rabbits were randonaly divided into three groups：Sham-operated control （SC） group,ischemia reperfusion （IR） group （4 hours of bilateral hindlimb ischemia and 4 hours of reperfusion）, and ischemic preconditioning （IPC） group （ three cycles of 5-minute ischemia followed by 5-minute reperfusion prior to IR）. At the end of the experiment, the contents of superoxide dismutase （SOD）, glutathione peroxidase （GSH-PX）, xanthine oxidase （XOD） activity,MDA and NO in lung tissue were detected. Meanwhile, lung index was measured and the pathological change in the lung was observed. Results： In the IR group,NO content and the activities of SOD and GSH-PX of the lung tissue were significantly decrease; the XOD activity, MDA level and lung index of the lung tissue were obviously increased. In the IPC group, the changes of the parameters mentioned above were obviously attenuated. Conclusion： Limb IPC protects the lung from the injury associated with limb ischemia-reperfusion via elevating the functions of anti-oxidation anti-peroxidative reaction of lipids.

关 键 词：缺血预处理 肺 再灌注损伤 抗氧化系统 

分 类 号：R658[医药卫生—外科学] R743.31[医药卫生—临床医学]