一步免疫印迹法同时检测抗RA33抗体和抗ENA抗体及其对RA患人的诊断价值  

The use of one-step immunoblotting to detect both anti-RA33 and anti—ENA antibodies,and its diagnostic value for RA patients

作　　者：刘学明[1] 于秀明[1] 于雅婷[1] 侯云峰[1] 孙然[1] 田园[1] 

机构地区：[1]长春中医学院附属医院,吉林长春130021

出　　处：《中国实验诊断学》2005年第5期754-756,共3页Chinese Journal of Laboratory Diagnosis

基　　金：吉林省科技厅科技发展计划项目(吉科合字第970576号)

摘　　要：目的提高抗RA33抗体诊断类风湿性关节炎(RA)的特异性。方法建立一步免疫印迹法,可同时检测抗RA33抗体和抗ENA抗体。并对266例不同风湿性疾病患者血清和24例正常人血清进行了检测。结果抗RA33抗体阳性率:RA 38%(38/100),SLE 35.8%(24/67),MCTD 36.4%(8/22),SS 8.3%(3/36)。抗RA33抗体阳性血清中抗ENA抗体的检出率:95.8%(23/24)SLE有抗Sm/u1RNP抗体,100%(8/8)MCTD有抗u1RNP抗体,100%(3/3)SS有抗SSA/SSB抗体,RA血清抗ENA抗体均为阴性。结论本文建立的一步免疫印迹法可有效地鉴别抗RA33抗体阳性的RA和SLE、MCTD等病人。Objective To improve the diagnostic specificity of anti-RA33 antibody for rheumatoid arthfitis（RA）. Methods Establishment of one-step immunoblotting technique to detect both anfi-RA33 and anti-ENA antibodies simultaneously, and 266 sera from different rheumatic patients were detected. Results The positive rate of anfi-RA33 antibody was 38.0% （38/100） in RA, 35.8%（24/67） in SLE, 36.4%（8/22） in MCTD, 8.3%（3/36） in SS respectively. Among these anti-RA33 positive sera, anti-Sm/ulRINP bands were detenninated in 95.8% （23/24） SLE, 100% （8/8） MCTD respectively, and anti-SSA/SSB bands were determi- nated in 100%（3/3） SS sere. In contrast, none of the anfi-ENA bands above were found in RA sere. Conchusion The one-step im- munblotting in this study is extremely useful to serologically differentiate anti-RA33 positive RA from anti-RA33 positive SLE and MCTD patients.

关 键 词：类风湿关节炎 RA33抗体 免疫印迹法 ENA抗体 

分 类 号：R593.22[医药卫生—内科学]