线粒体型天门冬氨酸氨基转移酶同工酶测定及其应用价值  

Determination of mitochondria aspartate aminotransferase(mAST) with inhibition immunoenzyme assay and its application value

作　　者：孙步彤[1] 盛传伦[1] 关勇[1] 孙淑艳[2] 荣墨克[1] 

机构地区：[1]吉林大学中日联谊医院,吉林长春130033 [2]吉林大学第一医院

出　　处：《中国实验诊断学》2005年第5期770-772,共3页Chinese Journal of Laboratory Diagnosis

摘　　要：目的建立免疫抑制法测定各类心脏和肝脏疾病的m-AST,观察其应用价值.方法提取纯化心肌细胞质型天门冬氨酸氨基转移酶同工酶(s-AST)酶蛋白,免疫羊制备抗人s-AST抗体,应用免疫抑制原理抑制标本中的s-AST活性,测定m-AST活性.结果血清m-AST参考值4～15u/L,急性心肌梗死60～310、陈旧心肌梗死10～20、无梗死冠心病7～15、无心衰7～18、心衰8～25、心包炎10～16、心肌炎18～30 u/L;急性肝炎56～410、慢迁肝炎12～17、慢活肝炎18～90、有腹水肝硬化18～50、无腹水肝硬化12～30、原发性肝癌11～64、继发性肝癌16～95、肝脓肿15～21 u/L.有坏死的心、肝病m-AST明显升高.非心肝性恶性肿瘤和其他疾病基本在参考值范围内.结论测定m-AST可协助判定心、肝疾病的严重性和有无坏死.Objective To determine m-AST with inhibition immunoassay in patients with a variety of cardiac and hepatic diseases and to evaluate its clinical value. Methods We extracted and purified cardiac cytoplasmic aspartate aminotransferase（s-AST） apeenzyrae, then immunized sheep to produce antibody against human s-AST. The activity of m-AST was detennined with inhibition immunoassay, which can inhibit the activity of s-AST in the specimen. Results Reference value of serum m-AST is 4 - 15 u/L, while the value in acute cardiac infarction was 60 - 310, obsolete cardiac infarction 10 - 20, coronary heart disease without infarction 7 - 15, non-heart failure 7 - 18, heart failure 8 - 25, pericarditis 10 - 16, and myeearditis 18 - 30 u/L; the value in acute hepatitis was 56 - 410, chronic persisting hepatitis 12 - 17, chronic active hepatitis 18 - 90, cirrhosis with ascites 18 - 50, cirrhosis without ascites 12 - 30, primary hepatocareinoma 11 - 64, secondary hepatocareinoma 16 - 95, and hepatic abscess 15 - 21 u/L. The value of m-AST in patients with necrotic cardiac and hepatic diseases increased significantly, while values in non-cardiac or hepatic malignancies and other diseases are among reference value basically. Conclusion Determination of m-AST can help assess severity in cardiac and hepatic diseases and whether or not necrosis exists.

关 键 词：天门冬氨酸氨基转移酶 线粒体天门冬氨酸氨基转移酶同工酶 酶蛋白 酶免疫抑制法 

分 类 号：R446.6[医药卫生—诊断学]