吡格列酮对血管内皮细胞一氧化氮和内皮素分泌的影响  

Effects of Pioglitazone on the Secretion of Nitric Oxide and Endothelin-1 in Culture Vascular Endothelial Cells

作　　者：刘礼斌[1] 陈洲[2] 刘小莺[1] 张闿珍[1] 

机构地区：[1]福建医科大学附属协和医院内分泌科,福建省内分泌研究所福州350001 [2]福建医科大学药学院药理学系,福州350004

出　　处：《福建医科大学学报》2005年第4期370-372,共3页Journal of Fujian Medical University

基　　金：福建省科技厅科研基金资助项目(99-Z-154)

摘　　要：目的观察吡格列酮(Pio)对血管内皮细胞一氧化氮(NO)和内皮素1(ET-1)分泌的影响.方法不同浓度Pio处理体外培养的血管内皮细胞(ECV304)72 h,硝酸还原酶法和放射免疫法测定细胞上清液内NO和ET-1水平.结果(1×10-8～1×10-5)mmol/LPio可显著促进ECV304细胞NO的释放和抑制其ET-1分泌,作用呈浓度依赖性.结论Pio可促进血管内皮细胞NO释放和抑制其ET-1分泌,这可能是Pio血管保护作用的机制之一.Objective To evaluate the effects of pioglitazone（Pio） on the secretion of nitric oxide and endothelin-1 in vascular endothelial cells. Methods Cultured vascular endothelial cells（ECV304） were incubated with various concentration pioglitazone. The levels of NO and ET-1 in the supernatants of the cultures were assayed by nitrate reductas method and RIA after 72 hours pretreated. Results The levels of NO in the supernatant of Pio treated group（concentration from 1 × 10^-8 to 1 ×10^-5 mmol/L） were higher than these of control group. But the levels of ET-1 in the supernatant of culture cell with same Pio concentration were lower than control group. Conclusion Pio can promote NO release and inhibit ET-1 release, all these maybe one of the mechanisms of Pio effect in vascular protect.

关 键 词：吡格列酮 血管内皮细胞 一氧化氮 内皮素 

分 类 号：R96[医药卫生—药理学]