Phalloidin对钙离子载体A23187诱导的人精子顶体反应的作用  

Phalloidin Plays an Important Role in Calcium Ionophore A23187-induced Human Acrosome Reaction

作　　者：吴帅[1] 王新生[2] 孟凡会[3] 

机构地区：[1]青岛市人民医院泌尿外科,山东青岛266003 [2]青岛大学医学院附属医院泌尿外科,山东青岛266003 [3]青岛大学医学院附属医院生殖医学中心,山东青岛266003

出　　处：《中华男科学杂志》2005年第11期835-837,共3页National Journal of Andrology

摘　　要：目的:探讨F-肌动蛋白在人精子顶体反应(AR)过程中的重要作用及可能机制。方法:将每例标本分为A、B、C、D、E 5组,共30例。以链球菌溶血素O(SLO)、Phalloid in、钙离子载体A23187分别处理各实验组人精子,其中A:A23187 3μmol/L;B:Phalloid in 40μmol/L+A23187 3μmol/L;C:SLO 0.5 U/m l+A23187 3μmol/L;D:SLO0.5 U/m l+Phalloid in 40μmol/L+A23187 3μmol/L;E:不添加任何试剂。分别采用罗丹明标记山豌豆凝集素(Rhodam ine-PSA,10μg/m l)荧光染色检测其AR,计算各组AR发生率(AR%)。结果:在5个实验组中,除A组与B组比较差异无显著性(P>0.05)外,其余各组两两比较差异均具有显著的统计学意义(P<0.01)。结论:Phalloid in对质膜完整的人精子AR发生率无影响。而在添加SLO的人精子模型中,Phalloid in较对照组能显著降低人精子AR发生率,提示F-肌动蛋白的聚合作用对人精子AR的发生具有重要意义,并且主要发生在精子顶体的内部。Objective： To disscuss the important role of actin polymerization in calcium ionophore A23187-induced human acrosome reaction and its mechanism. Methods： Each spermatozoon specimen was divided into five groups, treated with A23187 3μmol/L in Group A, Phalloidin 40 μmol/L and A23187 3 μmol/L in Group B, SLO 0.5 U/ml and A23187 3 μmol/L in Group C, SLO 0.5 U/ ml, Phalloidin 40 μmol/L and A23187 3 μmol/L in Group D, and nothing added in Grpup E. Then the percentage of the human acrosome reaction was assessed with Rhodamine-PSA（ 10 μg/ml）. Results： The difference of the human spermatozoon acrosome reaction was significant （ P 〈 0.01 ） among the 5 groups with or without SLO, Phalloidin and calcium ionophore A23187 but not between Groups A and B （P 〉 0.01 ）. Conclusion： Phalloidin does not work on the acrosome reaction of intact human spermatozoa, but in an SLO- permeabilized human spermatozoal model, it can obviously decrease the percentage of human spermatozoon acrosome reaction, which indicates that the polymerization of actin plays an important role in the course of human spermatozoon acrosome reaction, and mostly acts on the acrosome inside. Natl J Androl,2005 ,11 （11） ：835-837

关 键 词：精子肌动蛋白 链球菌溶血素O PHALLOIDIN 顶体反应 

分 类 号：R321[医药卫生—人体解剖和组织胚胎学]