血管内皮祖细胞移植提高裸鼠缺血皮瓣存活率  

Experiment of augmenting the survival areas of ischemic flap by transplanting endothelial progenitor cells

作　　者：易成刚[1] 郭树忠[1] 张琳西[1] 伍锦华[1] 张曦[1] 胡强[1] 张旭东[1] 周庆红[1] 

机构地区：[1]第四军医大学西京医院整形外科中心,西安710032

出　　处：《中华整形外科杂志》2005年第6期429-432,共4页Chinese Journal of Plastic Surgery

基　　金：国家自然科学基金项目青年科学基金资助项目(30400471);第四军医大学西京医院科技创新基金资助项目(XJCX03M010)

摘　　要：目的探讨血管内皮祖细胞(endothelialprogenitorcell,EPC)移植促进早期断蒂的缺血皮瓣的血管新生,从而提高皮瓣存活率的可能性。方法体外分离、培养人脐血中EPCs,免疫组织化学进行鉴定,然后移植于裸鼠随意皮瓣,皮瓣早期断蒂,裸鼠分两组:实验组(EPCs移植)、对照组(M199注射),术后皮瓣4d断蒂。观察皮瓣的存活率、激光多普勒血液监测仪监测血流灌注、CD34免疫组织化学检测皮瓣毛细血管密度、激光共聚焦检测EPCs在皮瓣内的密度。结果脐血中分离培养的EPCs表达CD34、KDR及CD133,实验组EPCs移植裸鼠皮瓣后,整合到缺血部位新生血管中,与对照组的皮瓣存活率分别为(60.3±2.1)%、(34.2±1.8)%(P<0.05);而且两组毛细血管密度、血流灌注差异均有统计学意义(P<0.05);实验组术后第7、11天时二组皮瓣中的EPCs密度分别为(75.2±6.5)个/mm2、(305.4±26.5)个/mm2,而对照组均为0个/mm2(P<0.05)。结论脐血中的EPCs体外培养后移植体内可促进缺血皮瓣的血管新生,提高存活率。Objective To investigate the feasibility of transplanted endothelial progenitor cells to ischemic flap with increased neovascularization and augmented the survival areas. Methods EPCs were isolated from human cord blood,cultured in vitro,identified by immunohistochemistry, Then EPCs were transplanted to ischemic flaps of 9 nude mice＇s back （experimental group）, and 9 nude mice＇s back flaps was injected with M199（control group）. And pedicle division time was 4 days after operation. CM-DiI was used to trace the transplanted cells. The blood perfusion of flaps was monitored by the laser Doppler flowetry, and the capillary density of flaps was detected by CD34 immunohistochemistry. Results EPCs expressed cell markers CD34, KDR and CD133. Transplanted EPCs survived and was incorporated into the capillary networks in the ischemic flaps of nude mice. The percent of experimental group＇ s flap survival area was （60.3 ± 2.1）% , significantly higher than the control group[ （34.2 ± 1.8） %, P 〈 0.05]. The blood perfusion,capillary density of flaps of experimental group was significantly higher than the control group （ P 〈 0.05）. Conclusions EPCs from human cord blood can increase ischemie flaps neovascularization and augment the survival areas.

关 键 词：血管内皮祖细胞 皮瓣 血管新生 

分 类 号：R622[医药卫生—整形外科]