六价铬对大鼠肝细胞体外的毒作用  

Cytotoxicity of Hexavalent Chromium to Rat Hepatocytes in Vitro

作　　者：王晓峰[1] 傅文宇[1] 朱欣[1] 徐立红[1] 

机构地区：[1]浙江大学医学院生物化学与分子生物学教研室

出　　处：《癌变．畸变．突变》2005年第6期343-345,共3页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家自然科学基金(No.20137010)

摘　　要：背景与目的:了解六价铬[Cr(VI)]对肝细胞引起的毒性作用。材料与方法:用Cr(VI)浓度为0、0.1、1、10及100μmol/L的溶液作用于大鼠肝细胞BRL_3A,以四甲基偶氮唑蓝(3_[4,5_dimethylthiazol_2_yl]_2,5diphenyl tetrazolium bromide,MTT)法检测细胞存活率及流式细胞术分析细胞凋亡率。结果:随着Cr(VI)浓度的升高细胞活性有降低趋势并且Cr(VI)所导致的细胞凋亡率也与其浓度存在正相关关系。结论:Cr(VI)可引起肝细胞的损伤,导致细胞凋亡的发生。BACKGROUND ＆ AIM： To study the toxicity of hexavalent chromium on rat hepatocytes .MATERtAL METHODS： Rat hepatocytes BRL- 3A were treated with hexavalent chromium at concentrations of 0, 0.1, 1, 10 and 100 μmol/L Viability was assessed by 3-[4, 5-dimethylthiazol-2-yl]-2, 5 diphenyl tetrazolium bromide（MTT） method and flow cytometry was used to analyze cell apoptosis. RFSULTS： Viability decreased with the increasing concentration of hexavalent chromium. Cell apoptosis induced by hexavalent chromium also showed in a dose-dependent pattern. CONCLUSION： These data indicated that hexavalent chromium had a damaging effect on hepatocytes and induced apoptosis at the concentrations used in the present study.

关 键 词：Cr(Ⅵ) 肝细胞 毒性 

分 类 号：R995[医药卫生—毒理学]