流式细胞仪检测X射线诱导小鼠骨髓红细胞微核率的初步研究  

Study on X-ray induced frequency of micronucleated erythrocytes in mice bone marrow by flow cytometer

作　　者：胡冰冰[1] 杨录军[1] 刘胜学[1] 曹佳[1] 

机构地区：[1]第三军医大学军事预防医学院卫生毒理学教研室,重庆400038

出　　处：《第三军医大学学报》2005年第22期2232-2234,共3页Journal of Third Military Medical University

基　　金：国家杰出青年基金资助项目(30125037);全军杰出青年基金资助项目(01J014)~~

摘　　要：目的观察经X射线照射后不同时相点小鼠骨髓红细胞微核率的变化,探讨嗜多染红细胞微核率(fMN-PCE)流式细胞仪(FCM)检测法能否作为快速检测辐射后早期遗传损害的生物计量仪。方法辐照组采用直线加速器激发X射线,对小鼠进行一次性全身照射,每组4只,剂量分别为0.5、3.0、6.0 Gy,于照后6、12、24 h取材;阴性对照组施以假照射,阳性对照组注射环磷酰胺。结果0.5 Gy X射线组受照后24 h,骨髓fMNPCE显著升高(P<0.05);而3.0 Gy及6.0 Gy组受照后6 h,fMNPCE显著升高(P<0.01)。结论红细胞微核流式细胞仪自动化检测法有作为辐射生物计量仪的应用前景。Objective To detect the frequency of micronucleated polychromatic erythrocytes （fMNPCE） in mice bone marrow after irradiated with X-ray by flow cytometer （FCM） to study whether fMNPCE detecting method could become a rapid biodosimeter for early radiation damage. Methods BALB/c mice were randomly assigned to receive the X-ray irradiation at dose of 0.5,3.0, 6.0 Gy （ n = 4 for each irradiation dose） , and the bone marrow was collected in 6, 12, 24 h after irradiation. Another 4 mice were intraperitoneally injected with cyclophosphamide twice as positive control, of which the bone marrow was collected in 42 h after intoxication began and 4 treated with spurious radiation as normal control. Results fMNPCE in mice bone marrow increased remarkably in 24 h after exposure to 0.5 Gy X-ray （P 〈 0.05） , and in 6 h after exposure to 3.0 Gy and 6.0 Gy X-ray （P 〈0.01 ）. Conclusion As one of biodosimeter for radiation, FCM automatic method is applicable to detect the frequency of micronucleated erythrocytes.

关 键 词：X射线 嗜多染红细胞 微核 流式细胞仪 辐射生物剂量仪 

分 类 号：R322.71[医药卫生—人体解剖和组织胚胎学] R446.9[医药卫生—基础医学]