碱性成纤维细胞生长因子和部分脱蛋白骨对兔股骨头缺损再血管化的影响(英文)  

Revascularizing effect of basic fibroblast growth factor and partially deproteinised bone on femoral head defects in rabbits

作　　者：龚跃昆[1] 彭吾训[1] 李晓辉[1] 李世和[1] 李彦林[1] 杨浩[1] 

机构地区：[1]昆明医学院第一附属医院骨科,云南省昆明市650032

出　　处：《中国临床康复》2005年第42期137-139,i0004,共4页Chinese Journal of Clinical Rehabilitation

基　　金：云南省教育厅科学基金项目(02XY251,03Z496C,04Z855C)~~

摘　　要：背景:股骨头缺血坏死的治疗是骨科领域的一大难题。然而,近来的研究表明碱性成纤维细胞生长因子是一有效的血管生成因子,推测其在股骨头缺血坏死的治疗方面可能具有一定潜能。目的:模拟临床上治疗股骨头坏死的开窗法的手术过程制备兔股骨头缺损,并植入碱性成纤维细胞生长因子和部分脱蛋白骨,以探讨碱性成纤维细胞生长因子对股骨头缺损的再血管化作用。设计:随机对照实验。单位:昆明医学院动物实验中心。材料:实验于2002-07/2003-07在昆明医学院动物实验中心完成。成年雄性健康新西兰白兔27只,体质量2.2～2.8kg。随机分为碱性成纤维细胞生长因子/部分脱蛋白骨复合骨组,单纯部分脱蛋白骨组,空白对照组,每组9只。方法:①制备碱性成纤维细胞生长因子/部分脱蛋白骨复合骨:含碱性成纤维细胞生长因子10ng/mm3载体。②股骨头缺损模型的建立及修复:取兔27只,在股骨头颈交界处开窗,建立骨缺损模型。复合骨组植入碱性成纤维细胞生长因子/部分脱蛋白骨;部分脱蛋白骨组植入单纯部分脱蛋白骨;空白对照组不植入任何植入物。实验动物分别于术后2,4,8周各时相点麻醉状态下进行墨汁灌注并处死,取材。主要观察指标:①兔股骨头标本组织学检查及血管计数。②微血管面积图像分析。结果:27只兔均进入结果分析。①兔股骨头标本组织学检查及血管计数:术后8周,复合骨组:移植材料被骨组织所取代,骨髓腔形成,其中有丰富的髓内血管存在;脱蛋白骨组:移植材料亦被骨样组织包裹,且大部分部分吸收;空白对照组:股骨头的缺损区被纤维组织填充,在缺损区周边的结缔组织中可见新生骨样组织和散在软骨细胞岛,血管数量较少。2周微血管计数复合骨组均显著高于部分脱蛋白骨组和空白对照组[(31.833±7.914)比(22.917±2.079)和(11.250±4.220)(血管数量/视野),P<0.01,BACKGROUND：The treatment of avascular necrosis of femoral head remains a clinical challenge. However, recent study shows that basic fibroblast growth factor （Bfgf） is an effective angiogenic growth factor. We suppose that Bfgf has the potential in treating avascular necrosis of femoral head through revascularizing effect and steogenesis.OBJECTIVE： To develop a rabbit model that simulates the clinical situation with trapdoor bone rafting and evaluate the revascularizing effect in the repair of femoral head defect with Bfgf/partially deproteinised bone （PDPB）.DESIGN： A completely randomized controlled trial.SETTING： Animal Experimental Center of Kunming Medical College.MATERIALS： The experiment was conducted in the Animal Experimental Center of Kunming Medical College between July 2002 and July 2003.Totally 27 adult male healthy New Zealand rabbits, weighing 2.2 to 2.8 kg,were recruited and randomly divided into Bfgf/composite PDPB group,single PDPB group, and blank control group with 9 rabbits in each group.INTERVENTIONS： ① Preparation of Bfgf/PDPB： containing Bfgf 10 ng/mm3. ② Establishment and repair of femoral head defect model：Totally 27 rabbits were chosen. Bone defect model was stablished by opening a trapdoor between femoral head and femoral neck. Bfgf/PDPB was implanted in composite group; single PDPB was implanted in PDPB group; no implants were given in blank control group. The rabbits were killed after injection through blood vessels with prepared Chinese ink 2, 4and 8 weeks after operation, and then the femoral head of each rabbit was taken out as specimen.MAIN OUTCOME MEASURES： ① Histological examination and blood vessel counting. ② Image analysis of microvessel area.RESULTS： Twenty-seven rabbits all entered the result analysis. ① Histological examination and vessel count of femoral head specimens in rabbits：8 weeks after operation, composite bone group： implants were replaced with bone tissues and medullary cavity of bone formed. A lot of medullary vessels we

关 键 词：股骨头坏死 成纤维细胞生长因子 内皮生长因子 

分 类 号：R681.6[医药卫生—骨科学]