复合庆大霉素羊同种抗菌骨抗感染及成骨实验  

Anti-infection and osteosis of gentamicin-impregnated ovine bone allografts

作　　者：张育敏[1] 李宝兴[2] 赵亚平[2] 李幼忱[2] 康悦[2] 殷少芳[2] 赖炽香[2] 

机构地区：[1]南方医科大学组织工程研究中心,广东省广州市510515 [2]中国辐射防护研究院生物材料与制药技术研究所,山西省太原市030006

出　　处：《中国临床康复》2005年第42期31-33,共3页Chinese Journal of Clinical Rehabilitation

摘　　要：目的:研制一种既有成骨作用又有局部抗感染能力,且有较低免疫原性的新型高效骨植入材料。方法:实验于2002-09/2005-03在中国辐射防护研究院动物实验中心完成,采用超声负压双重复合法制备庆大霉素含量为172mg/条的复合羊同种抗菌骨,植入羊股(肱)骨骨缺损合并感染模型绵羊5只(实验组),对照组5只植入空白骨,采用大体观察、白细胞计数、影像学、组织学、细菌学检查等指标评价其抗感染效果及成骨能力。结果:参加实验10只绵羊,对照组1只羊于第17天死于金黄色葡萄球菌所致败血症。进入结果分析为9只绵羊。①实验组除1例感染外,其余有效的控制了感染并有新骨形成。②对照组术后第4,6周植入骨被脓液包裹,第8,10周植骨区基本被纤维组织填充。③成骨组织及炎性组织体积密度上差异均存在统计学意义(P<0.01),说明实验组比对照组有较多的新骨形成与较轻微的炎性反应。④实验组与对照组在骨组织细菌密度差异有显著性意义[(1.64±3.37)×108,(1.64±3.27)×1012CFU/g,P<0.05]。说明庆大霉素复合抗菌骨能有效抑制局部细菌的生长繁殖。结论:复合庆大霉素同种抗菌骨具有较好的局部抗感染效果及成骨能力。AIM： To study and prepare a new kind of bone graft （gentamicinimpregnated bone） with local anti-infective function, low immunogenicity and high osteosis. METHODS： The experiment was carried out in the animal experimental center of China Institute for Radiation Protection from September 2002 to March 2005. The gentamicin-compound bone ovine allografts with 172 mg gentamicin per strip were prepared by means of ultrasonic and vacuum, and the bone allografts without gentamicin were implanted into the ovine model of contaminated defects （n=5）. Blank bone was implanted in the control group （n=5）. Gross observation, leucocyte count, radiological, microbial and histological investigations were carried out to evaluate the anti-infective and osteosis capability. RESULTS： Ten sheep were used in the study, 1 in the control group died from hematosepsis caused by staphylococcus aureus, finally 9 sheep were involved in the analysis of results. ① In the gentamicin treatment group, besides 1 sheep was infected, the others had a good anti-infection and osteosis. ② In the control group, the implanted bone were encapsulated in pus at 4 and 6 weeks, and filled with fibrous tissue at 8, 10 and 12 weeks after surgery. ③ The volume density was significantly different between the osteogenic tissue and inflammatory tissue （P 〈 0.01）, indicating that there were more new bone formation and milder inflammatory reactions in the gentamicin treatment group than in the control group. ④ The bacteria density of bone tissue was significantly different between the gentamicin treatment group and control group [（1.64±3.37）×10^8, （1.64±3.27）×10^12 CFU/g, P 〈0.05], suggesting that gentamicin-impregnated bone allograft could effectively inhibit the growth and breeding of local bacteria. CONCLUSION： Gentamicin-impregnated bone allograft is of a good antiinfective function and osteosis.

关 键 词：骨移植 移植 同种 庆大霉素类 骨疾病 感染性 

分 类 号：R63[医药卫生—外科学]