Ⅱ型胶原预防和治疗骨关节炎的动物实验  

Animal experiment in the prevention and treatment of osteoarthritis with type Ⅱ collagen

作　　者：梁佩红[1] 李斯明[1] 叶春婷[1] 邹海燕[1] 彭燕豪[1] 黄雪芳[1] 

机构地区：[1]暨南大学医学院附属第四医院(广州市红十字会医院)创伤外科研究所,广东省广州市510220

出　　处：《中国临床康复》2005年第42期88-89,共2页Chinese Journal of Clinical Rehabilitation

摘　　要：目的:采用H ulth法对新西兰兔建立膝骨关节炎动物模型,以透明质酸钠及注射用生理盐水为对照,观察Ⅱ型胶原注射治疗兔膝关节炎的愈合效果。方法:实验于2002-02/2003-07在暨南大学医学院附属第四医院完成。取36只新西兰兔制备Hulth膝骨关节炎动物模型,随机等分为3组:实验组术前及术后每两周关节内注射1m L1%Ⅱ型胶原至12周。关节内注射透明质酸钠组和关节内注射生理盐水组分别于术前及术后每两周关节内注射1%透明质酸钠1m L,生理盐水1m L,以上各组于术后4,8,12周各处死动物4只,作标本手术显微镜观察和组织学检测。结果:①实验组与透明质酸钠组骨关节炎改变较生理盐水对照组明显减轻。②术后4周时,实验组软骨膜光滑,软骨细胞排列整齐,可见双核软骨细胞;关节内注射透明质酸钠组软骨膜也光滑,软骨细胞排列整齐;关节内注射生理盐水组软骨膜细胞增生,有裂隙,软骨层变薄,细胞排列不规则。术后8周时,实验组软骨膜细胞增生,有裂隙,软骨细胞层次减少,但排列尚规则;关节内注射透明质酸钠组的光镜改变基本与实验组相似;关节内注射生理盐水组出现软骨表面缺损及纤维化,软骨细胞层薄排列不规则。术后12周时,实验组、关节内注射透明质酸钠组的光镜改变与实验组术后8周时相似;关节内注射生理盐水组在其术后8周时光镜改变基础上有更广泛的软骨缺损,部分切片可见软骨下骨质暴露。结论:①Ⅱ型胶原与透明质酸钠匀能有效抑制骨关节炎。②外源性Ⅱ型胶原可以替代透明质酸钠应用于骨关节炎的防治。AIM： Animal models with knee osteoarthritis in New Zealand rabbits were established with Hulth approach, taking hyaluronic acid natrium and saline used for injection as control, to observe the healing effects of collagen II by injecting in the treatment of knee arthritis in rabbits. METHODS： The experiment was conducted at the Fourth Affiliated Hospital, Medical College of Jinan University from February 2002 to July 2003. Thirty-six New Zealand Rabbits were used to make animal models of knee osteoarthritis and divided into three groups randomly. The rabbits in the experimental group were treated with 1 mL 1% collagen II with intra- articular injection every two weeks before operation and after operation till the 12^nd week. Those in the intra-articular injection hyaluronic acid natrium group and intra-articular injection saline group were treated with 1% hyaluronic acid natrium 1 mL and saline 1 mL before operation and after operation, respectively. Four animals in every group were killed at the 4^th, 8^th and 124 weeks to perform operation observed with microscope and the histology detection. RESULTS：① The osteoarthritis in the experimental group and hyaluronic acid natrium group significantly relieved as compared with the saline group. ② At the 4^th week after operation, the perichondrium was smooth and glossy in the experimental group, and the chondrocytes arranged in line. The double nucleus chondrocytes could be seen. The perichondrium was also smooth and glossy in the intra-articular injection hyaluronic acid natrium group. The chondrocytes arranged in line. In the intra-articular injection saline group with crevice, and the cartilages the perichondrium cells proliferated layers became thin with irregular arrangement. At the 8^th week after operation, in the experimental group the perichondrium cells proliferated with crevice, and the cartilages layers decreased, but the arrangement was regular. The light microscope changes in the intra-articular injection hyaluronic acid natrium group were simila

关 键 词：胶原Ⅱ型 骨关节炎 动物实验 

分 类 号：R684.3[医药卫生—骨科学]