镉和菲复合污染对土壤微生物DNA序列多样性的影响  

Effects of Cd and Phenanthrene on DNA Sequence Diversity of Soil Microbial Community

作　　者：张峦[1] 沈国清[1] 陆贻通[1] 曹林奎[1] 

机构地区：[1]上海交通大学资源与环境系,上海201101

出　　处：《科技通报》2005年第6期763-769,共7页Bulletin of Science and Technology

基　　金：国家基础研究发展计划973(2004CB418503);国家自然科学基金资助项目(200337010);上海市基础研究重点项目(04JC14051)

摘　　要：在室内培养条件下,采用RAPD(随机扩增多态)分子标记技术研究了重金属镉和多环芳烃菲复合污染对土壤中微生物群落DNA序列多样性的影响。结果表明,培养15d和30d,镉-菲单一和复合污染均导致土壤微生物群落DNA序列的丰富度、均匀度和多样性指数增加。实验证明,污染早期,镉和菲会引起土壤微生物DNA序列本身发生变化,其中镉-菲复合污染对土壤微生物DNA序列多样性的影响最大,与单一污染相比具有明显差异。Combined effects of cadmium and phenanthrene on the DNA sequence diversity of soil microbial community was evaluated by random amplified polymorphic DNA （RAPD） fingerprints in the controlled condition. The results showed that the DNA sequence richness ,diversity index and evenness increased in the soil contaminated by cadmium and phenanthrene alone or together within 15 and 30 days. These influences was significantly in combined pollution soil. It was suggested that DNA sequences of microbial communities may be changed in the soil contaminated by cadmium and phenanthrene at early stage of pollution.

关 键 词：镉 菲 RAPD 土壤微生物 DNA多样性 

分 类 号：X503[环境科学与工程—环境工程]