HPLC法测定穴贴定喘膏中木兰脂素的含量  

HPLC Determination of Magnolin in Xuetie Dingchuan Plaster

作　　者：倪美华[1] 狄留庆[1] 孙淑萍[1] 

机构地区：[1]南京中医药大学新药及海洋药物研究开发中心,南京210029

出　　处：《中药新药与临床药理》2005年第6期433-435,共3页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：江苏省教育厅高新技术产业化课题(编号JH02-103)

摘　　要：目的建立穴贴定喘膏中有效成分木兰脂素的含量测定方法。方法采用HPLC法,色谱柱为LichrospherC8柱(4.6m m×250mm,5μm),柱温30℃,检测波长为278nm,流速1mL m/in,流动相为乙腈-1.55%四氢呋喃水溶液(34∶66)。结果木兰脂素在0.42～5.25μg范围内与峰面积呈良好线性关系,相关系数r=0.9999,贴膏中木兰脂素的平均回收率为100.50%,RSD=2.22%(n=9)。结论该方法准确、简便、稳定,能有效地控制该制剂的质量。Objective To establish a HPLC method for the determination of magnolin in Xuetie Dingchuan Plaster. Methods The HPLC chromatographic condition was as follows： the analytical column was Lichrospher C8 （4.6 mm×250mm, 5μm）, the mobile phase consisted of A （acetonitrile） ： B （1.55% tetrahydrofuran-water） 34： 66, the flow rate was 1.0 mL·min^-1, the detected wavelength was 278 nm, and the column temperature was 30℃. Results The calibration curve was linear （r=0.9999） in the range of 0.42-5.25μg for magnolin, the average recovery of the method was 100.50%, with RSD 2.22% （n=9）. Conclusion The method is accurate, simple and stable, and can be used for the quality control of Xuetie Dingchuan Plaster.

关 键 词：穴贴定喘膏 木兰脂素 高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学]