番鸭呼肠病毒的分离与RT-PCR鉴定  

Isolation and Identification of Muscovy duck reovirus by RT-PCR

作　　者：林锋强[1] 胡奇林[1] 欧阳岁东[1] 陈仕龙[1] 朱小丽[1] 程晓霞[1] 

机构地区：[1]福建省农业科学院畜牧兽医研究所,福建福州350003

出　　处：《动物医学进展》2005年第11期64-65,共2页Progress In Veterinary Medicine

基　　金：福建省自然科学基金项目(B0010028);福建省科技项目(2001Z061)

摘　　要：从发病番鸭中分离到1株病毒,用该病毒接种番鸭胚,至第2代可引起鸭胚死亡,胚体出血,部分鸭胚肝脾有少量白点。分离毒经用RT-PCR方法进行检测,可与番鸭呼肠病毒标准株扩增出大小一致的特异条带,证实该分离毒为番鸭呼肠病毒。A strain of virus isolated from dead ducks was inoculated into the allantoic cavity of muscovy duck embryos, showing that it caused death to the embryos at the second generation, the embryonic body hemorrhaged, and white spot presented on the liver and spleen of some embryos. The isolated virus was compareed with standard virus by RT-PCR in which both amplified specific band. It indicated that the isolated virus was a strain of Muscovy duck reovirus.

关 键 词：番鸭呼肠病毒 分离 鉴定 

分 类 号：S852.659.4[农业科学—基础兽医学] Q503[农业科学—兽医学]