糖尿病大鼠视网膜细胞凋亡的影响因素  

Influencing factors of apoptosis in the retinal tissue of diabetic rats

作　　者：何剑峰[1] 杜娟[2] 唐柳萍[2] 李向荣[2] 黄艳霞[2] 

机构地区：[1]广西医科大学第一附属医院眼科,广西壮族自治区南宁市530021 [2]广西医科大学第五附属医院眼科

出　　处：《中国临床康复》2005年第39期72-74,共3页Chinese Journal of Clinical Rehabilitation

基　　金：广西自然科学基金资助(0007036)~~

摘　　要：目的:探讨能够影响糖尿病大鼠视网膜组织中细胞凋亡的因素。方法:实验于2003-03/2004-03在中山大学医学动物实验中心完成。选取无菌级成年纯系雄性Wistar大鼠40只,禁食12h后随机分成糖尿病组和对照组,20只/组。糖尿病组腹腔一次性注射链脲佐菌素65mg/kg诱发糖尿病,对照组腹腔注射等量柠檬酸盐缓冲液。于糖尿病组发病后4,6,12,16周用酶联免疫分析法检测两组大鼠视网膜细胞凋亡程度,硫代巴比妥酸荧光法检测两组大鼠视网膜组织中脂质过氧化物含量,化学发光法检测两组大鼠视网膜组织中超氧化物歧化酶的活性,并与空腹血糖、糖化血红蛋白、血中自由基含量及糖尿病病程进行相关分析。组间比较行t检验,组内比较行H检验,各指标间的相关性采用双变量相关分析。结果:实验纳入大鼠40只,均进入结果分析。①两组大鼠发病前后不同时期空腹血糖的变化:两组大鼠在发病前基本相近[(4.16±1.1),(4.16±1.2)mmol/L,P>0.05],发病后4,6,12,16周糖尿病组均显著高于对照组(t>5.1679,P<0.001)。②两组大鼠发病后4,6,12,16周各项指标检测结果比较:与对照组比较,糖尿病组糖化血红蛋白、全血中自由基含量、视网膜组织中脂质过氧化物水平、视网膜细胞溶解液吸光值均明显增高(t=2.4388,P<0.05;t>2.5673,P<0.01;t>2.8514,P<0.01;t>2.9155,P<0.01),而视网膜组织中超氧化物歧化酶活性显著降低(t>2.9612,P<0.01)。③各指标间相关性分析:糖尿病组视网膜细胞凋亡量与空腹血糖浓度、糖化血红蛋白水平、血中自由基含量、视网膜组织中过氧化脂质水平和糖尿病病程呈高度正相关(r=0.7584,0.7844,0.7962,0.8248,0.6246,P均<0.001),而与视网膜组织中超氧化物岐化酶呈高度负相关(r=-0.8617,P<0.001)。结论:糖尿病大鼠发病后4周即发现血中自由基含量增加、视网膜组织中抗氧化机能下降、同时伴有视网膜细胞凋亡。糖尿病大鼠�AIM： To investigate the factors that influence the apoptosis in the retinal tissue of diabetic rats. METHODS： The experiment was carried out in the experimental center of medical animal of Sun Yat-sen University between March 2003 and March 2004. Forty adult pure male Wistar rats were randomly divided into two groups after fasted for 12 hours： diabetic group （n=20） and control group （n=20）. The rats in the diabetic group were induced to develop diabetes by once intraperitoneal injection of streptocotocin （65 mg/kg）, and those in the control group were treated with intraperitoneal injection of saline of citrate buffer of the same volume. At 4, 6, 12 and 16 weeks after diabetic attack, the severity of apoptosis in the retinal tissue was deteced with enzyme-linked immunoassay （EIA） in both groups, the content of lipid peroxidation in the retinal tissue was detected with the thiobarbituric acid fluorescent assay, the activities of superoxide dismutase ih the retinal tissues were detected by chemiluminescence. The relations of the apoptosis with levels of fasting blood glucose, glycosylated hemoglobin, the content of free radicals in blood and the disease course were also analyzed. The t test and H test were applied in the intergroup and intragroup comparisons respectively, the relation between the indexes was analyzed with bivariate correlation analysis. RESULTS： All the 40 rats were involved in the analysis of results. ① The changes of fasting blood glucose before and after the diabetic attack between the two groups： The levels of fasting blood glucose before attack were close [（4.16±1.1）, （4.16±1.2） mmol/L, P 〉 0.05], but all significantly higher in the diabetic group than in the control group at 4, 6, 12 and 16 weeks after attack （t 〉 5.167 9, P 〈 0.001）. ②Comparison of the indexes at 4, 6, 12 and 16 weeks after attack between the two groups： Compared with the control group, the content of glycosylated hemoglobin, content of free radicals in whole blood, level of

关 键 词：糖尿病 视网膜 细胞凋亡 大鼠 

分 类 号：R587.1[医药卫生—内分泌]