高压氧对糖尿病大鼠血清和肾脏转化生长因子β_1表达的影响  

Effects of hyperbaric oxygen on expression of transforming growth factor-beta 1 in serum and kidney in diabetes rats

作　　者：刘芳[1] 周景花[2] 管佩玲[2] 彭名淑[3] 张杰[4] 钟慈声[5] 施佳[1] 许剑锋[1] 邵贵强[2] 卜国强[2] 姚庆姑[1] 

机构地区：[1]复旦大学附属上海市第五人民医院内分泌科,上海市200240 [2]复旦大学附属上海市第五人民医院高压氧室,上海市200240 [3]复旦大学附属上海市第五人民医院免疫室,上海市200240 [4]复旦大学附属上海市第五人民医院病理科,上海市200240 [5]复旦大学上海医学院电镜室,上海市200032

出　　处：《中国临床康复》2005年第39期78-80,i0002,共4页Chinese Journal of Clinical Rehabilitation

基　　金：上海市市科委项目(014119053)~~

摘　　要：目的:观察高压氧治疗对糖尿病大鼠血清和肾脏转化生长因子β1表达及肾脏超微结构的影响。方法:①实验于2002-10/2003-03在上海市第五人民医院动物房、病理科和复旦大学医学院电镜室完成。选用2月龄雄性健康SD大鼠90只。随机留取正常大鼠20只,其余大鼠禁食过夜后,按55mg/kg,腹腔内注射10g/L的链脲佐菌素溶液造成糖尿病模型。糖尿病大鼠病程2个月后经过电镜病理观察证实糖尿病大鼠糖尿病肾病的超微结构异常63只。随机分为3组,模型组(n=32)和高压氧治疗组(n=31)及对照组(n=20)。对照组(n=20):仅腹腔注射等体积生理盐水,不进行干预。模型组(n=32):隔天清晨皮下注射一次正规胰岛素和鱼精蛋白锌胰岛素控制血糖在8～13mmol/L。高压氧治疗组(n=31):同样用胰岛素和鱼精蛋白锌胰岛素控制血糖,每天进入动物舱在0.15MPa的压力下,吸入95%以上浓度的纯氧1h。连续20d。②分别在高压氧治疗10和20d后分批留取股动脉血和肾脏后处死大鼠。用快速血糖仪测定尾静脉血糖,并测定体质量。取股动脉血测定各组大鼠血清转化生长因子β1水平,同时取肾脏进行苏木精-伊红染色病理学观察和电镜观察,并用转化生长因子β1多抗对肾组织做3’,3-二氨基联苯胺二步法免疫组化染色。③组间比较用t检验。结果:①在分组干预过程中,高压氧治疗组死亡1只,模型组死亡2只。进行血清转化生长因子β1水平测定大鼠:对照组20只、高压氧治疗组30只、模型组30只。高压氧治疗10d后,对照组处死6只,模型组处死7只,高压氧治疗组处死7只大鼠;治疗20d后,对照组处死6只,模型组处死8只,高压氧治疗组处死8只大鼠。分别进行相应指标测定。②治疗前,糖尿病大鼠血清转化生长因子β1水平明显高于对照组(P<0.05);病理学主要表现为肾小球内玻璃样变、硬化和血管减少。电镜观察显示模型组肾小球血管袢足突�AIM： To investigate the effects of hyperbaric oxygen therapy （HBOT） on the serum and expression of transforming growth factor-beta 1（TGF-β1） with ultrastructure of kidney in diabetic rats. METHODS： ① The experiment was conducted at the Animal Ward, Department of Pathology, Shanghai Municipal Fifth People＇s Hospital and Electron-microscope Room, Shanghai Medical University, Fudan University from October 2002 to March 2003. Ninety male healthy SD rats aged 2 months were selected. Twenty normal rats were retained randomly. Based on 55 mg/kg, other fasting rats were treated with 10 g/L streptozotocin solution with intraperitoneal injection as diabetes model overnight. Among the diabetes rats, there were 63 diabetic nephropathy rats with abnormal uhrastructure after 2-months progress observed with electron microscope. They were assigned randomly into 3 groups： model group （n=32）, hyperbaric oxygen treatment group （n=31） and control group （n=20）. The 20 rats in the control group were only treated with saline at the same volume with intraperitoneal injection, without intervention. The 32 rats in the model group were treated with regular insulin and depesulin once in the other morning to control glucose from 8 to 13 mmol/L. The 31 rats in the hyperbaric oxygen treatment group were equally treated with insulin and deposulin to control glucose, entering animal chamber at the pressure of 0.15 MPa, sucking^ure oxygen at the concentration of 95% for 1 hour, lasing for 20 days. ② After being treated with hyperbaric oxygen for 10 and 20 days, respectively, femoral arterial blood and kidney were gained in batch, and then the rats were killed. Caudal vein glucose and body mass were detected with swift glucose apparatus. The femoral arterial blood was gained to detect the level of TGF-β1 of serum in rats of every group. At the same time, the pathological change of kidney was observed using HE staining and electron-microscope, and the TGF-β1 immunostaining in kidney was observed using DAB two-step

关 键 词：高压氧/治疗应用 糖尿病肾病/治疗 转化生长因子Β 

分 类 号：R587.1[医药卫生—内分泌]