稳恒磁场对小鼠肝脏组织谷胱甘肽过氧化物酶活性及丙二醛含量的影响及其强度效应(英文)  

Effects of static magnetic field on the activity of glutathione peroxidase and malondialdehyde content in mouse hepatic tissue

作　　者：刘方平[1] 吴全义[1] 陆任云[2] 王卉放[2] 端礼荣[3] 

机构地区：[1]江苏大学医学物理教研室,江苏省镇江市212001 [2]江苏大学生物化学教研室,江苏省镇江市212001 [3]江苏大学公共卫生教研室,江苏省镇江市212001

出　　处：《中国临床康复》2005年第39期168-169,共2页Chinese Journal of Clinical Rehabilitation

摘　　要：背景:丙二醛是脂质过氧化的终产物,丙二醛含量可以推断机体内的脂质过氧化损伤情况;谷胱甘肽过氧化物酶是生物机体内的自由基清除剂。而稳恒磁场对生物体的正、负面效应目前尚无定论。目的:探讨稳恒磁场对小鼠肝脏组织抗氧化能力的影响及其强度效应。设计:观察对比实验。单位:江苏大学医学物理实验室、生化实验室。材料:实验于2003-01/12在江苏大学医学物理实验室及生化实验室完成。选择昆明种小鼠30只,雌雄各半,体质量18～20g。暴露装置采用铁氧体瓦形磁铁自制暴露盒。方法:将30只小鼠随机分成5组,正常对照组、磁感应强度(24.6±4.2),(42.0±2.1),(63.5±3.0),(85.1±2.9)mT组各6只。每天定时将正常对照组小鼠放入无磁场暴露盒内,磁感应强度(24.6±4.2),(42.0±2.1),(63.5±3.0),(85.1±2.9)mT组小鼠分别置于以上4种不同强度的稳恒磁场暴露盒内2次,2h/次,15d后麻醉处死小鼠,检测肝脏组织中谷胱甘肽过氧化物酶活性及丙二醛含量。主要观察指标:各组小鼠肝脏组织中丙二醛含量及谷胱甘肽过氧化物酶活性。结果:30只小鼠全部进入结果分析,无脱失。①小鼠肝脏组织中丙二醛含量:磁感应强度(24.6±4.2)mT,(42.0±2.1)mT组小鼠显著低于正常对照组[(12.70±0.53,12.96±0.72,17.62±0.91)μmol/g(F=10.4,9.89,P<0.01)]。②小鼠肝脏组织中谷胱甘肽过氧化物酶活性:磁感应强度(24.6±4.2)mT,(42.0±2.1)mT组小鼠显著高于正常对照组[(143.36±8.34,150.69±12.00,87.51±11.34)μkat/g(F=10.0,11.3,P<0.01)]。结论:一定磁感应强度的稳恒磁场能降低小鼠肝组织的丙二醛含量,增加谷胱甘肽过氧化物酶活性,提高抗氧化酶的活力,降低过氧化脂质的生成,减少其对生物体的损害,对延缓衰老有积极作用。BACKGROUND： As the end product of lipid peroxidation, malondialdehyde （MDA） content can be used for assessment lipid peroxidation injury.Glutathione peroxidase （GSH-Px） acts as a free radical scavenger. Currently the effect of static magnetic field on the organism, whether positive or negative, has not been elucidated.OBJECTIVE： To study the effect of static magnetic field on anti-oxidation capacity of mouse hepatic tissues and its intensity dependence for producing such effects.DESIGN： A controlled comparative experiment.SETTING： Laboratories of Medical Physics and Biochemistry of Jiangsu University.MATERIALS： The experiment was conducted in the Laboratories of Medical Physics and Biochemistry of Jiangsu University from January to December 2003. Totally 30 mice of either sex weighing 18-20 g were selected and subjected to magnetic filed exposure using a self-designed ferrite magnet apparatus.METHODS： The mice were equally randomized into normal control group and 4 exposure groups exposed to magnetic field of （24.6±4.2） mT,（42.0±2.1） mT, （63.5±3.0） mT, and （85.1±2.9） mT, respectively. The mice in the 4 exposure groups were exposed to static magnetic field of the specified intensity for 2 hours twice a day, while those in the normal control group were subjected to the sham exposure apparatus without magnetic field at scheduled time points every day. After 15 days of exposure, the mice were sacrificed and the GSH-Px activity and the MDA content in the hepatic tissue were assayed.MAIN OUTCOME MEASURES： GSH-Px activity and MDA content in hepatic tissue of the mice.RESULTS： Thirty mice entered the final analysis without losses. MDA content in （24.6±4.2） mT and （42.0±2.1） mT groups were obviously lower than that in the normal control group [（12.70±0.53）, （12.96±0.72）, and （17.62±0.91） μmol/g, respectively, F=10.4, 9.89, P 〈 0.01]. The GSH-Px activity in the hepatic tissue in （24.6±4.2） mT and （42.0±2.1） mT groups were obviously higher tha

关 键 词：电磁场 谷胱甘肽过氧化酶 丙二醛 

分 类 号：R358.2[医药卫生—基础医学]