卡托普利对压力负荷增加大鼠左室重构的逆转作用(英文)  

Reversing effect of captopril on left ventricular remodeling in rats with increased pressure load

作　　者：蔡辉[1] 胡婉英[2] 王艳君[3] 郭郡浩[1] 胡兵[1] 姚茹冰[1] 

机构地区：[1]解放军南京军区南京总医院中西医结合科,江苏省南京市210002 [2]上海中医药大学附属曙光医院,上海市200021 [3]河北医科大学博士后流动站,河北省石家庄市050017

出　　处：《中国临床康复》2005年第39期170-172,共3页Chinese Journal of Clinical Rehabilitation

摘　　要：背景:压力负荷可以导致肾素血管紧张素醛固酮系统激活,进而导致左室重构,卡托普利对这种重构具有逆转作用。目的:观察卡托普利逆转压力负荷增加大鼠左室重构的作用及其机制。设计:随机对照实验。单位:南京军区南京总医院中西医结合科,上海中医药大学附属曙光医院,河北医科大学博士后流动站。材料:试验于1998-01/12在上海中医药大学实验室完成。选取Wistar雄性大鼠153只,共做5次试验,只有第4次试验是选择9只大鼠,其余试验均为36只大鼠。方法:将每次试验所选取的36只Wistar雄性大鼠随机分成假手术组、模型组、鹿角方组3组。模型组造模4周后,双蒸水灌胃15mL/kg,1次/d,连续4周。假手术组只分离腹主动脉,不用银夹狭窄,4周后,同模型组。卡托普利组造模4周后,以卡托普利100mg/kg,1次/d,用双蒸水15mL/kg稀释后灌胃,连续4周。主要观察指标:卡托普利对大鼠左室质量指数、Ⅰ型胶原和Ⅲ型胶原mRNA的表达,血浆和心肌血管紧张素Ⅱ含量、血浆心钠素和血清醛固酮含量的影响。结果:153只大鼠均进入结果分析。在检测Ⅰ型胶原积分吸光度试验中假手术组死亡和卡托普利组各3只,模型组死亡1只,最终纳入29只大鼠。在检测Ⅲ型胶原积分吸光度试验中,假手术组死亡3只,模型组死亡4只,卡托普利组死亡2只,最终纳入27只大鼠。均因动物手术损伤而脱失。①左室质量指数比较:假手术组和卡托普利组明显低于模型组[(2.24±0.12)/1000,(2.67±0.40)/1000,(3.15±0.47)/1000,t=2.649,6.499,P<0.001,0.05]。②Ⅰ型胶原积分吸光度的改变:假手术组和卡托普利组明显低于模型组[(0.57±0.19,0.86±0.25,2.79±2.00),t=3.661,3.170,P<0.01,0.05]。③Ⅲ型胶原积分吸光度的改变:假手术组和卡托普利组明显低于模型组[(0.48±0.10,0.52±0.29,0.84±0.27),t=3.560,2.417,P<0.01,0.05]。④Ⅰ型胶原mRNA的表达:假手术组和卡托普利组明显低于�BACKGROUND： Pressure overload can lead to the activation of reninangiotensin-aldosterone system, and then left ventricular remodeling occurs.Captopril can reverse this progress.OBJECTIVE： To observe the effect of captopril in reversing left ventricular remodeling in rats with pressure overload and to elucidate its possible mechanism.DESIGN： A randomized controlled trial.SETTING： Department of Integrated Traditional Chinese Medicine and Western Medicine, Nanjing General Hospital （） Nanjing Military Area Command of Chinese PLA; Shuguang Hospital affiliated to Shanghai University of Traditional Chinese Medicine; Postdoctor Institute of Hebei Medical University.MATERIALS： The experiment was conducted in the Laboratory of Shanghai University of Traditional Chinese Medicine between January 1998 and October 1998. Totally 153 male Wistar rats were recruited.There were 5 experiments, 36 rats in each experiment except 9 rats in the fourth one.METHODS： A total of 36 Wistar male rats selected for each experiment were randomly divided into sham operation group, model group and captopril group. Rats in model group received modeling for 4 weeks, then gastric perfusion with 15 mL/kg of distilled water once per day for 4 consecutive weeks. Abdominal aorta of the rats in sham-operation group were separated without closure by sliver clip, then 4 weeks later the procedure was the same as that in model group. In captopril group, 4 weeks after modeling, the rats were performed gastric perfusion with diluted captopril of 100 mg/kg, once a day for 4 consecutive weeks.MAIN OUTCOME MEASURES： The effect of captopril on left ventricular mass index （LVMI）, mRNA expression of collagen type Ⅰ and Ⅲ, content of plasma angiotensin Ⅱ （Ang Ⅱ ） and left ventricular tissue Ang Ⅱ,plasma atrial natriuretic factor （ANF）, and serum aldosterone （ALD） level.RESULTS： Totally 153 rats entered the result analysis. During the test for detecting the absorbance （A） of collagen type Ⅰ, 3 rats in sham operation

关 键 词：胶原Ⅰ型 胶原Ⅲ型 基因表达 卡托普利 

分 类 号：R540.47[医药卫生—心血管疾病]