复方黄精口服液对心力衰竭大鼠心肌肌球蛋白重链变化的作用(英文)  

Effect of compound huangjing oral liquid on myocardial myosin heavy-chain in rats with heart failure

作　　者：陈金水 吴天敏 林圣远 杜建 吴可贵 王华军 陈小明 

机构地区：[1]福建省医科大学附属第一医院中医科,福建省福州市350003 [2]福建省中医学院,福建省福州市350001 [3]福建省高血压研究所,福建省福州市350003

出　　处：《中国临床康复》2005年第39期190-192,共3页Chinese Journal of Clinical Rehabilitation

摘　　要：背景:阿霉素是蒽环类抗肿瘤药,对多种恶性肿瘤有抑制作用。但由于其毒性大,可引起与剂量依赖性的心脏毒性,严重者可致心力衰竭。复方黄精口服液能抗自由基损伤,有望用于心力衰竭的辅助治疗。目的:探讨复方黄精口服液抗心力衰竭疗效及其作用机制。设计:随机对照观察。单位:福建省医科大学附属第一医院中医科、福建省中医学院及福建省中医学院。材料:实验于2000-08/2001-05在福建省高血压研究所完成,选用66只雄性SD大鼠。方法:采用阿霉素损伤大鼠心肌致心力衰竭为模型,66只大鼠随机分为6组,每组11只。正常组:腹腔注射等体积生理盐水。阿霉素组:于实验开始后第2,4天腹腔注射阿霉素1mg/kg;第6,8天腹腔注射2mg/kg;第10,12天腹腔注射3mg/kg;第14,16天腹腔注射4mg/kg,16d累计用药量达20mg/kg。阿霉素+复方黄精口服液2mL(小剂量组)。阿霉素+复方黄精口服液4mL(中剂量组)。阿霉素+复方黄精口服液6mL(大剂量组)。小、中、大剂量组于实验开始后分别每日用复方黄精口服液灌胃。阿霉素的剂量用法同阿霉素单用组。阿霉素+天保宁(天保宁组):天保宁450mg/kg隔日灌服共8d。阿霉素的剂量用法同阿霉素单用组。主要观察指标:观察各组大鼠心系数,α-肌球蛋白重链,β-肌球蛋白重链的变化。结果:纳入实验动物数66只,在整个实验期间阿霉素组有5只大鼠,小剂量组4只大鼠,中剂量组2只大鼠,大剂量组3只大鼠,天保宁组3只大鼠死于明显的充血性心力衰竭,进入结果分析47只。与正常组比较,阿霉素组的α-肌球蛋白重链下降20.88%(P<0.01),β-肌球蛋白重链上升50.93%(P<0.01),心系数升高33.83%(P<0.01)。经给药治疗后,心肌β-肌球蛋白重链向α-肌球蛋白重链转化,与阿霉素组比较,各药物治疗组α-肌球蛋白重链上调(P<0.01),β-肌球蛋白重链下降(P<0.01),心系数均有不同程度下降(P<0.01或P<0.05),以上�BACKGROUND： Adriamycin is anthracycline-based drugs of anti-cancer and inhibits many malignant tumors. But due to the large toxicity, it will induce dose-dependent cardiac toxicity, resulting in heart failure in severe case. Compound huangjing oral lipid is against the injury of free radial and is expected to be applied as an assistant therapy for heart failure.OBJECTIVE： To probe into the therapeutic effect of compound huangjing oral lipid and its mechanism on heart failure.DESIGN： Randomized controlled observation was designed.SETTING： Department of Traditional Chinese Medicine , First Affiliated Hospital of Fujian University of Medical Science, Fujian College of Traditional Chinese Medicine.MATERIALS： The experiment was performed in Fujian Research Institute of Hypertension from August 2000 to May 2001, in which, 66 rats were employed and randomized into 6 groups, 11 rats in each one.METHODS： In normal group, physiological saline of equal volume was injected abdominally. In adriamycin group, adriamycin 1mg/kg was injected abdominally on the 2nd and 4th days after experiment, 2 mg/kg was injected on the 6th and 8th days, 3 mg/kg was on the 10th and 12th days and 4 mg/kg was on the 14th and 16th days. The dose was accumulated up to 20 mg/kg in 16 days. In adriamycin＋compound huangjing oral liquid 2 mL （small-dose group）, adriamycin ＋compound huangjing oral liquid 4 mL （moderate-dose group） and adriamycin＋compound huangjing oral liquid 6ml （large-dose group）, the oral lipid of various doses was applied for gastric perfusion everyday successively from the beginning of experiment, in which, the dose of adriamycin was same as adriamycin group. In adriamycin＋tebonin group （tebonin group）, tebonin 450 mg/kg was administrated once every two days, totally for 8 days, in which, the dose of adriamycin was same as adriamycin group.MAIN OUTCOME MEASURES： To observe the changes of Left ventricular weight and body weight （LVW/BW）, α-MHC （myosin heavy-chain）and β-MHC.RESULTS： I

关 键 词：黄精 口服液 阿霉素 心力衰竭 充血性 肌球蛋白重链 

分 类 号：R541[医药卫生—心血管疾病]