SARS并发肺纤维化和骨缺血性坏死患者血清生物标志物的检测  

The detection of serum biomarkers in patients with pulmonary fibrosis and bone avascular necrosis after SARS-Cov infection

作　　者：吴优[1] 曾家伟[1] 何冰[1] 魏茂提[1] 苏彬[1] 杨震[1] 杜海科[1] 付维林[1] 王世鑫[1] 

机构地区：[1]武警医学院附属医院,天津300162

出　　处：《武警医学院学报》2005年第6期444-447,F0002,F0004,共6页Acta Academiae Medicinae CPAPF

基　　金：天津市重大科技攻关重点项目(05YFSZSF02900)

摘　　要：【目的】检测SARS并发肺纤维化和骨缺血性坏死患者血清生物标志物。【方法】利用表面加强激光解析电离飞行时间质谱(Surface Enhanced Laser Desorption Ionization-Time Of Flight-Mass Spectrometry,SELDI-TOF-MS)技术研究SARS康复人员的血清蛋白质组学变化,用Ciphergen ProteinChip软件和BioMarker Wizard软件分析蛋白质组数据。【结果】SARS并发肺纤维化和骨缺血性坏死的康复人员血清中有3个特异性分子稳定上调,质荷比(M/Z)分别为5 023、8 606和10 641。与正常人血清蛋白质谱相比,SARS人群3个差异性蛋白质相对含量较高(P=0.02)。【结论】SARS并发肺纤维化和骨缺血性坏死患者可能存在血清生物标志物。[Objective] To detect serum biomarkers in patients with pulmonary fibrosis and bone avascular necrosis after SARS-Cov infection. [Methods] Surface enhanced laser desorption ionizatlon-time of flight-mass spectrometry （SELDI- TOF-MS） was used to analyze the serum samples of SARS patients with pulmonary fibrosis and bone avascular necrosis after SARS-Cov infection. The data were analyzed by Ciphergen ProteinChip and BioMarker Wizard software. [Results] Three specific serum proteins were detected upregulation in the serum of SARS patients compared with the control group and the M/Z of them were 5023, 8606 and 10641 respectively. And the relative concentration of each protein is significantly higher than that of control population （ P = 0.02）. [Conclusion] There are maybe some specific serum biomarkers in patients with pulmonary fibrosis and bone avascular necrosis after SARS-Cov infection.

关 键 词：SARS SELDI-TOF-MS 血清 生物标志物 

分 类 号：R563.1[医药卫生—呼吸系统]