高致病性H5亚型禽流行性感冒病毒血凝素单克隆抗体的制备与初步应用  

Development and Characterization of MAb against Haemagglutinin of Highly Pathogenic H5 Avian Influenza Virus

作　　者：陈毅歆[1] 罗海峰[1] 葛胜祥[1] 郭永利[1] 彭耿 王嘉[3] 陈鸿霖[3] 张军[1] 管轶[3] 夏宁邵[1] 

机构地区：[1]厦门大学细胞生物学与肿瘤细胞工程教育部重点实验室福建省医学分子病毒学研究中心,厦门361005 [2]北京万泰生物药业有限公司,北京102200 [3]汕头大学医学院汕港联合流感中心,汕头515031

出　　处：《病毒学报》2005年第6期422-427,共6页Chinese Journal of Virology

基　　金：教育部跨世纪优秀人才培养计划(2002);福建省科技重大专项(2004YZ01-1)

摘　　要：以禽流感病毒株Ck/HK/Yu22/02(H5N1)作为免疫原,利用常规杂交瘤技术和血凝抑制试验法成功地筛选出6株稳定分泌抗高致病性H5亚型禽流感病毒血凝素的单克隆抗体(单抗),分别命名为2F2、3C8、3FC1、7C6、10HD4和13G4。经血凝抑制试验法分析,结果发现这6株单抗具有特异性高、反应性强、识别谱宽且互补等特点。基于单抗2F2,初步建立了三种H5N1病毒诊断方法,经评估证实均具有很好的特异性。由此说明,研究制备的抗H5亚型禽流感病毒血凝素单抗可适用于H5N1病毒的诊断。Six MAbs against haemagglutinin of higly pathogenic H5 avian influenza virus were developed by fusing SP2/0 myeloma cells with spleen cells of BALB/c mice immunized with Ck/HK/Yu22/02（HSN1） , which were designated as 2F2, 3C8, 3FC1, 7C6, 10HD4 and 13G4 respectively. By hemagglutinin inhibition test （HI）, these six MAbs displayed high specificity and reactivity. Based on the MAb 2F2, we developed three diagnostic assays for HSN1 viruses, all displaying very good specificity to HSN1 virus. The results suggested that these MAbs will be very useful in diagnosing HSN1 viruses.

关 键 词：禽流行性感冒病毒H5N1 血凝素 单克隆抗体 酶联免疫吸附试验 胶体金免疫试验 

分 类 号：R373.17[医药卫生—病原生物学]