栀子药材中三种有效成分HPLC定量分析结果不确定度的评定  

Estimation of Measurement Uncertainty of Analytical Results for the Determination of Three Active Components from Gardenia jasminoides Ellis.by HPLC

作　　者：胡震[1] 王义明[2] 罗国安[2] 贺浪冲[1] 

机构地区：[1]西安交通大学医学院,西安710061 [2]清华大学化学系,北京100084

出　　处：《中药材》2005年第11期991-994,共4页Journal of Chinese Medicinal Materials

摘　　要：采用HPLC方法同时测定栀子药材中栀子苷、绿原酸和藏红花素Ⅰ的含量,通过对测试方法流程进行分析,确定不确定度来源,结合含量测定方法验证数据,计算了不确定度分量、合成不确定度及扩展不确定度;结果表明,用HPLC方法定量分析栀子药材中栀子苷、绿原酸和藏红花素1的方法满足现代药物分析方法学的要求,且三者的扩展不确定度分别为0.1024,0.2254,0.1265。Objective： To estimate the uncertainty for quantitative analysis results of geniposide, chloregenic acid and crocinl Ⅰ in Gardenia jasminoides Ellis. Methods： HPLC method was employed to determine the amounts of geniposide, choloregenic acid and crocinl Ⅰ in Gardenia jasminoides Ellis. Analyzing the uncertainty sources arising from the procedure of analysis, the standard uncertainty and combined uncertainty and expanded uncertainty were calculated according to the data of HPLC. Results： This method met the requirements of modem pharmaceutical analysis, and the expand uncertainties for the HPLC method of the three components are 0. 1024, 0. 2254,0. 1264 ,respectively. Conclusion： Applying measurement uncertainty to the evaluation of quantitative analysis results of active components in Gardenia jasminoides Ellis. is an improvement to the actual error evaluation system.

关 键 词：测量不确定度 高效液相色谱法 栀子 定量分析 

分 类 号：R764.04[医药卫生—耳鼻咽喉科] R284.1[医药卫生—临床医学]