EGb对脑外伤大鼠ICAM-1及其基因表达作用的实验研究  

Effects of Extract of Ginkgo biloba on Intercellular Adhesion Molecule-1 and mRNA Expression in Rats with Cerebral Injury

作　　者：张荣军[1] 游潮[1] 蔡博文[1] 贺民[1] 唐建建[1] 刘展[1] 焦庆芳[1] 

机构地区：[1]四川大学华西医院神经外科,成都610041

出　　处：《中药材》2005年第11期1009-1012,共4页Journal of Chinese Medicinal Materials

摘　　要：目的:研究银杏叶提取物(EGb)对脑外伤大鼠脑组织细胞间黏附分子-1(ICAM-1)及其mRNA表达的影响,以探讨EGb脑保护的作用与机理。方法:72只SD大鼠随机分为对照组、小剂量、大剂量银杏叶提取物治疗组,使用Feeney氏法建立大鼠闭合性脑损伤模型,通过免疫组织化学、图像分析和原位杂交等方法观察细胞间黏附分子-1及其mRNA的表达情况。结果:细胞间黏附分子-1阳性细胞位于坏死中心与正常皮层之间的交界区,脑外伤6 h即出现细胞间黏附分子-1蛋白及其mRNA表达,24 h表达到高峰。银杏叶提取物治疗组细胞间黏附分子-1表达与对照组比较明显减少(P<0.01)。结论:银杏叶提取物可明显减少交界区神经细胞的损伤,其对神经元的保护作用可能与抑制细胞间黏附分子-1及其mRNA的表达有关。银杏叶提取物可减轻大鼠脑缺血损伤,减少细胞间黏附分子-1表达及中性粒细胞浸润。Objective： To investigate the effects of Extract of Ginkgo biloba on ICAM-1 and mRNA expression in rats with cerebral injury, and discuss its protective mechanism. Methods ： The acute closed brain injury model was set up in rats according Feeney＇ s method. Seventy-two rats were randomly divided into four groups. The levels of ICAM-1 expression were observed using immunohistochemistry and computerized imaging technique and the expression of mRNA were studied using RT-PCR techniques. Results： ICAM-1 positive cells located in the transitional zone between the necrotic core and normal cortex. ICAM-1 protein and mRNA expression began to show 6 hours after cerebral injury, peaked at 24 hours. As compared with the model group, ICAM-1 and mRNA expression was significantly reduced in the group EGb （P 〈0. 01 ）. Conclusion： EGb can markedly reduce the injury of nerve cell in the transitional zone, alleviate rat cerebral injury, and decrease ICAM-1 and mRNA expression and neutrophils infiltration. The protection may be related with its inhibition on ICAM-1 and mRNA expression.

关 键 词：银杏叶提取物 细胞间黏附分子-1 脑损伤 中性粒细胞 血管内皮细胞 

分 类 号：R827.14[医药卫生—临床医学] R743.31