PCR-RFLP鉴别假高粱和丝克高粱  

Differentiation of Sorghum halepense and S. silk by PCR-RFLP method.

作　　者：郭琼霞[1] 黄可辉[1] 虞赟[2] 

机构地区：[1]福建出入境检验检疫局,福州350003 [2]福建农林大学

出　　处：《植物检疫》2005年第6期330-333,共4页Plant Quarantine

基　　金：国家质量检验疫监督总局2002年科技项目

摘　　要：本文利用PCR特异引物扩增rDNA-18S-26S基因,对PCR产物进行纯化、克隆和测序;分析假高粱与丝克高粱rDNA-18S-26S的酶切图谱,选择合适的限制性内切酶对两者PCR产物进行特异性切割,按照酶切片段的长度来区分两个近似种。被AflⅢ酶切后,假高粱被切割为674bp、174bp两个片段,而丝克高粱被切割成181bp、174bp、495bp、669bp4个片段。为建立PCR-RFLP分子鉴定方法有效地区分假高粱与丝克高粱这两个种提供依据。To establish a molecular method for diffecentiation of Sorghum halepense and S. silk, polymerase chain reaction-restriction fragment length polymorphism analysis was used. The rDNA-18S-26S gene was amplified by specific primers and PCR products were purified, cloned , sequenced and analyzed. AfllII restriction enzyme was chosen based on the restriction maps of rDNA-18S-26S gene of S. halepense and S. silk to cut PCR products. Results showed that two different rDNA-18S-26S sequences blasted as S. halepense and S. silk were detected in the study. S. halepense and S. silk can be visuallly distinguished according to the electrophoresis pattern： the four fragments in 181bp, 174bp, 495bp and 669bp were observed in the lanes of S. silk while only two bands in 674bp and 174bp for S. halepense. Therefore, this PCR-RFLP method established in this study can be used as the basis for identification between S. halepense and S. silk.

关 键 词：假高粱 丝克高粱 分子鉴别 PCR产物酶切片段长度多态性分析 

分 类 号：S451[农业科学—植物保护]