聚乳酸表面处理的AFM观察及对细胞粘附性的影响  

CHANGES IN SURFACE MORPHOLOGY OF POLY (L-LACTIC ACID) OBSERVED BY ATOMIC FORCE MICROSCOPY AND EFFECT ON CELL ADHESION

作　　者：王云起[1] 焦延鹏[2] 蔡继业[1] 周长忍[2] 

机构地区：[1]暨南大学化学系,广东广州510632 [2]暨南大学材料科学与工程系,广东广州510632

出　　处：《高分子材料科学与工程》2005年第6期181-184,188,共5页Polymer Materials Science & Engineering

基　　金：国家973项目(2001CB510101);国家自然科学基金资助项目(重点项目30230350面上项目60278014)

摘　　要：利用N aOH对溶液浇铸法制备的聚L-乳酸膜(PLLA)进行不同时间的表面处理,采用接触角仪和原子力显微镜(AFM)对处理前、后的材料表面进行亲水性和形貌表征,并初步研究了表面处理的材料对细胞粘附性的影响。结果表明,N aOH处理后的PLLA膜的亲水性明显改善,并且其表面平均粗糙度(Ra)由20 nm增加到40 nm^150 nm。成纤维细胞在改性后的材料表面的粘附和生长较改性前有了很大提高。Poly （L-lactic acid） （PLLA） films were fabricated by solution-casting method and subsequently their surfaces were modified by NaOH hydrolysis. The changes of surface properties were characterized by contact angles and atomic force microscopy （AFM）. The mouse L929 fibroblast cells were used as model cells to evaluate the cell adhesion of PLLA before and after surface treatment. The results show the hydrophilicity of PLLA films are improved after treatment and the surface roughness, presented with a roughness parameter, R. is increased from about 20 nm to among 40 nm and 150 nm. The adhesion and growth of mouse L929 fibroblasts on modified films are significantly improved. In conclusion, increased surface roughness of PLLA films at 40 nm～150 nm can significantly enhance cell affinity that can be useful for applications of tissue engineering.

关 键 词：聚L-乳酸 表面处理 原子力显微镜 细胞 粘附性 

分 类 号：TB39[一般工业技术—材料科学与工程]