生长因子与软骨细胞-胶原海绵复合体联合应用修复软骨缺损的研究  

Effect of basic fibroblast growth factor on the repair of articular cartilage defects through cartilage tissue engineering

作　　者：朱如里[1] 董英海[2] 高学纯[1] 王明丽[3] 卢晓林[1] 刘淑恒[1] 张之栋[1] 

机构地区：[1]安徽医科大学第一附属医院骨科 [2]上海第二医科大学附属仁济医院骨科 [3]安徽医科大学微生物教研室

出　　处：《中华老年医学杂志》2005年第11期847-850,共4页Chinese Journal of Geriatrics

基　　金：安徽省教委基金资助项目(NO99j10108)

摘　　要：目的研究在体条件下碱性成纤维生长因子(bFGF)与软骨细胞-胶原海绵复合体联合应用修复软骨缺损的疗效。方法取3日龄异体幼兔膝关节软骨细胞,体外培养后接种在胶原海绵支架上,再做移植。同一大白兔双侧膝关节内外髁分为4组:空白对照组、软骨细胞-胶原海绵复合体移植组(复合体移植组)、bFGF应用组和软骨细胞-胶原海绵复合体与bFGF联合应用组。移植术后不同时间分批处死,进行大体、光镜和电镜观察。结果移植术后12周联合应用组软骨缺损修复以完全修复为主,而复合体移植组以不完全修复为主,差异有统计学意义;20周时2组均得到修复,差异无统计学意义,组织学分析均为软骨组织;在不同时间点空白对照组和单纯bFGF应用组软骨缺损修复差异无统计学意义,但组织学上修复的组织,前者为纤维组织,而后者同时有软骨组织存在。结论bFGF在软骨组织工程中有促进软骨缺损早期修复的作用。Objective To explore the effect of basic fibroblast growth（ bFGF） combined with the collagen sponge-chondrocytes complex on the repair of articular cartilage defects through cartilage tissue engineering. Methods Chondrocytes which were taken from three days old New Zealand immature rabbits, were cultured in vitro, then were seeded onto the collagen sponge. The spongechondrocytes complex were transplanted into full-thickness defects . The rabbits were divided into four groups： control groups, collagen sponge-chondrocytes complex transplantation groups, bFGF-treated groups, bFGF combined with complex-treated groups. The rabbits were killed at different weeks of post-operation. The macroscopic, histologic and electron microscopic evaluations were performed. Results There were significant differences in the repair of defects between combined application groups and complex transplantation groups at 12 weeks after operaion. The former were superior to the latter. There were no differences at 20 weeks after operation. Typical cartilage tissue was formed. There were no differences between control and bFGF-treated groups in different time, but the repaired tissues were not the same： the former were covered with fibrous tissue, the latter with both fibrous tissue and cartilage tissue. Conclusions bFGF promotes the repair of articular cartilage defects in the cartilage tissue engineering.

关 键 词：成纤维生长因子2 软骨细胞 胶原Ⅰ型 关节软骨 

分 类 号：R683[医药卫生—骨科学]