用于丝网印刷电极条上几种固定酶方法的比较研究  

Comparative Study of Various Enzyme Immobilization Techniques on Screen-printed Electrodes

作　　者：张君[1] 郭伟[2] 袁倬斌[1] 

机构地区：[1]中国科学院研究生院应用化学研究所 [2]中国科学院生态环境研究中心,北京100085

出　　处：《分析测试学报》2005年第6期6-9,15,共5页Journal of Instrumental Analysis

基　　金："十五"科技攻关重大项目(2001BA210A04-7);国家自然科学基金资助项目(20175025);国家自然科学基金分析化学重点基金资助项目(20235010);电分析化学国家重点实验室基金资助

摘　　要：对几种酶固定方法:电极表面简单物理吸附、单分子层自组装固定酶法、光敏胶酶固定法、牛血清白蛋白-戊二醛分子交联固定法进行了比较研究,并把这几种方法用于乙酰胆碱酯酶在丝网印刷电极上的固定,在此基础上,提出了在丝网印刷电极上固定乙酰胆碱酯酶的新方法———由Nafion加固的蛋白-戊二醛分子交联法,实际使用中取得了很好的固定效果和良好的酶稳定性。用此法制成的生物传感器对敌敌畏农药进行了测定,经0.1 mg.kg-1农药抑制后,乙酰胆碱酯酶的活性被有效抑制(抑制率达到10%左右)。实验结果表明,Nafion-蛋白-戊二醛交联是一种行之有效的丝网印刷电极上的酶固定方法。Various enzyme immobilization techniques, namely the simple physical adsorption, the self assembling mono-layer, the sensitization enzyme immobilization, the bovine serum albumin（BSA） -glutaraldehyde cross-linking for the immobilization of acetylcholinesterase （ACE） on screen-printed electrodes for pesticide biosensor were compared. Based on the comparative results, a new Nation - BSA - glutaraldehyde cross-linking method was developed to immobilize ACE on screen-printed electrodes. The biosensor made by this novel method has excellent performances, and the enzyme was immobilized efficiently with good stability. The biosensor has been used to determine the dichlorvos, a virulent organophosphate pesticide. The result showed that the ACE activity was inhibited efficiently by 0.1 mg · kg^-1 pesticide, indicating that the new method was good for immobilizing ACE on the screen-printed electrodes.

关 键 词：生物传感器 丝网印刷 微电极 酶固定 

分 类 号：O657.15[理学—分析化学]