PbS-PAA复合纳米粒子共振光散射法测定蛋白质  

Determination of Proteins with PbS-PAA Composite Nanoparticle by Resonance Light Scattering Technique

作　　者：陈红旗[1] 马娟[1] 刁雪莲[1] 王伦[1] 

机构地区：[1]安徽师范大学化学与材料科学学院,安徽芜湖241000

出　　处：《分析测试学报》2005年第6期56-58,61,共4页Journal of Instrumental Analysis

基　　金：安徽省自然科学基金资助项目(03044904);国家自然科学基金资助项目(20375001;20575001);安徽师范大学青年科学基金资助项目(2005xqn16)

摘　　要：基于蛋白质对聚丙烯酸修饰的纳米PbS复合粒子共振光散射的增强效应,建立了一种新的测定蛋白质的共振光散射法.在超声辐射下,用过二硫酸钾(KPS)做引发剂,用原位聚合的方法使丙烯酸原位聚合在纳米PbS上.所得的复合纳米粒子表面就覆盖了大量的羧基(-COOH),不仅增加了复合纳米粒子的水溶性和稳定性,而且使其具有良好的生物相容性.在pH=0.91的NaAc-HCl溶液中,复合纳米粒子与蛋白质结合后,使得其共振光散射强度剧增,且其增强的程度与所加入的蛋白质浓度成线性关系.在λ=385 nm处,共振光散射强度最大.在最佳条件下,建立了工作曲线,线性范围为0.2～20μg·mL-1(HSA),0.2～20μg·mL-1(Human γ-IgG),0.15～18μg·mL-1(BSA);检出限分别为25、14、27ng·mL-1.该法简便、快速、灵敏度高.A novel resonance light scattering（RLS） method is developed for the determination of protein. It is based on the fact that the RLS intensity of nano-PbS modified by polyacrylic acid（PAA） is significantly increased in the presence of protein. The modified nano-PbS is prepared by polymerizing the acrylic acid （AA） onto the nano-PbS in the presence of potassium persulfate （KPS） by an in situ polymerization method under ultrasonic irradiation. The surface of the PBS - PAA composite nanoparticle is covered with abundant carboxylic groups（-COOH）. The composite nanoparticle is water-soluble and biocompatible. It can combine with protein in NaAc - HCI solution（pH = 0.91 ） to give strong RLS at λ = 385 nm. Under optimum conditions, the RLS intensity is linearly proportional to the concentration of proteins. The linear range is 0.2 - 20μg·mL^-1 for HSA, 0.2 - 20μg·mL^-1 for Human λ-IgG and 0.15 - 18μg·mL^-1 for BSA, and the LOD is 25 ng · mL^-1 for HAS, 14 ng · mL^-1 for Human λ-IgG and 27 ng · mL^-1 for BSA. The method presented here is simple, fast and sensitive.

关 键 词：共振光散射 复合纳米粒子 PBS 蛋白质 

分 类 号：O657.39[理学—分析化学]