以对苯二酚为底物伏安法测定辣根过氧化物酶  

VOLTAMMETRIC DETECTION OF HORSERADISH PEROXIDASE USING HYDROQUINONE AS SUBSTRATE

作　　者：王彤[1] 薛淑云[1] 段春生[1] 

机构地区：[1]青岛大学师范学院,山东青岛266071

出　　处：《精细石油化工》2005年第6期61-64,共4页Speciality Petrochemicals

摘　　要：提出了以对苯二酚作为辣根过氧化物酶(HRP)的底物,伏安法测定辣根过氧化物酶活性的新方法。在醋酸-酸酸钠缓冲溶液中,对于 HRP 催化 H_2O_2氧化对苯二酚的反应,其氧化产物在滴汞电极上可以发生还原反应,于—0.81 V(vs.SCE)处产生一个较为灵敏的伏安峰。此伏安峰可用于测定 HRP 的活性,测定 HRP的线性范围为8×10^(-10)～5×10^(-8)g/mL,检测下限为6×10^(-10)g/mL。同时,考察了酶催化反应条件和伏安测定条件。A new electrochemical method with hydroquinone as substrate for the analysis of horseradish peroxidase（HRP） activity was studied. In this method, the enzymecatalyzes the oxidation of the hydroquinone by H2O2 and the the electro-reduction reaction of the oxidation product produces a sensitive voltammetric wave at potential of -0. 81 V（vs. SCE） in HAc-NaAc buffer solution. In the detection of free HRP the detection range was 8 × 10^-10- 5 × 10^-8 g/mL and the detection limit was 6 × 10^-10 g/mL. The conditions for the enzymatic reaction and the voltammetric detection are optimized.

关 键 词：对苯二酚 辣根过氧化物酶 伏安法 免疫分析 

分 类 号：O657.1[理学—分析化学]