醛固酮上调大鼠组织钙调神经磷酸酶活性及抑制血浆一氧化氮水平  

Aldosterone Upregulates Calcineurin Activities in Vital Organs and Decreases Plasma NO Level in Rats

作　　者：陈雯[1] 谢晓华[1] 常连庆[1] 孙愚[1] 张颖[1] 牟丽华[1] 刘宁 刘秀华[1] 

机构地区：[1]解放军总医院南12病室,北京100853 [2]解放军272医院,天津300000

出　　处：《高血压杂志》2005年第11期721-724,共4页Chinese Journal of Hypertension

基　　金：本课题受"十五"军队医药卫生科研基金(02Z010)

摘　　要：目的 研究醛固酮对大鼠重要脏器心、脑、肾、脾、肺和肝脏中钙调神经磷酸酶(CaN)活性及血浆血管紧张素Ⅱ(AngⅡ)、内皮素(ET-1)和一氧化氮(NO)水平的影响，探讨醛固酮在CaN依赖的信号传导通路中的作用。方法 取21只雄性Wistar大鼠随机分为3组：醛固酮(Ald)组(腹腔内注射Ald 18μg／d 4周)、环孢霉素A(CsA)干预组(以Ald处理同时腹腔内给予CsA 5mg／kg·d注射共4周)及正常对照组。采用放免法测定血浆AngⅡ、Ald和ET-1浓度，采用比色测定NO浓度及大鼠各组织器官CaN活性。结果 Ald组大鼠血浆NO_3^-浓度较对照组明显下降(P＜0.05)，CsA干预后血NO_3^-浓度明显回升(P＜0.05)。各组AngⅡ、ET-1浓度无明显变化。Ald组大鼠心、脾、肾、肺、脑和肝脏中CaN活性较正常对照组分别上升147％、65％、38％、100％、43％和50％(均P＜0.05)，CsA干预显著缓解上述组织器官CaN活性的上升(P＜0.05)。结论 醛固酮能上调大鼠重要组织脏器CaN活性并抑制血浆NO水平，可能在激活CaN依赖的信号传导通路中具有重要作用。Objective To investigate the effect of aldosterone （Aid） on calcineurin （CaN） activities of the vital organs and plasma NO, Ang Ⅱ and ET-1 levels in rats and the role of aldosterone on the calcineurin-dependent signal transduction pathways. Methods Twenty-one male Wister rats were divided into 3 groups randomly： Aid group（n=7） with Aid 18 μg/d, i. p for 4 weeks; cyclosporine A（CsA） group（n= 7） with Aid ＋ CsA 5 mg/kg · d, i. p for 4 weeks; and contral group（n=7）. The plasma concentrations of Aid, angiotensin Ⅱ （Ang Ⅱ ）and endothelin（ET-1）were determined by the radioimmunoassay. The blood level of NO was assayed by the nitrate assay. CaN activity was measured by using PNPP as substrate. Results Compared with contral group, the tissue CaN activities in heart, spleen, kidney, lung, brain, liver of Aid rats was increased by 147%, 65%, 38%,100%, 43%, 50%, respectively （All P〈7 0.05） and the blood NO：（ concentration of Aid rats was decreased（P〈50.05）. Cyclosporine A reverses the increases of CaN activities induced by aldosterone in these organs and tissues（ P〈70.05 ） and increased the blood NO3- concentration. The plasma concentrations of Ang Ⅱ and ET-1 of three groups did not differ significantly. Conclusion Mediating the calcineurin-dependent signal transduction pathways, aldosterone upregulate calcineurin activity of the vital organs and decrease the plasma NO level of rats.

关 键 词：醛固酮 钙调神经磷酸酶 一氧化氮 环孢霉素A 

分 类 号：R33[医药卫生—人体生理学]