妊娠高血压综合征患者非浓缩尿蛋白电泳分析与临床意义  

Significance and analysis of unconcentrated urine sample from patients with pregnancy induced hypertension syndrome by protein electrophoresis

作　　者：赵小环[1] 吕时铭[1] 张艳[1] 郑斐[1] 

机构地区：[1]浙江大学医学院附属妇产科医院检验科,杭州310006

出　　处：《中华检验医学杂志》2005年第10期1041-1043,共3页Chinese Journal of Laboratory Medicine

基　　金：浙江省科技计基金划资助项目(2003C30047)

摘　　要：目的应用非浓缩尿蛋白电泳分析妊娠高血压综合征(妊高征)与正常妊娠妇女的尿蛋白成分特点,探讨尿蛋白成分与妊高征病情的相关性。方法采用十二烷基磺酸钠-琼脂糖凝胶电泳(SDS-AGE)检测114例妊高征患者(妊高征组)和110例正常晚期妊娠妇女(对照组)非浓缩尿蛋白成分。结果妊高征组尿中共检测到11种蛋白成分;正常对照组共检测到4种蛋白成分,以白蛋白为主。妊高征组尿蛋白成分构成与正常对照组比较,差异有显著统计学意义,P<0·05。尿蛋白成分构成与妊高征严重程度之间呈正相关,相关系数为0·587,P<0·01。妊高征病例尿中IgG和转铁蛋白的相对百分含量与妊高征程度之间呈正相关(rs=0·754,rs=0·697),P<0·01。结论妊高征者尿蛋白成分构成比正常孕妇复杂,尿蛋白成分与妊高征病情相关,SDS-AGE尿蛋白电泳分析有助于鉴别正常妊娠期生理性蛋白尿与妊高征病理性蛋白尿,也有助于妊高征病情评估。Objective To investigate the relationship between proteinuria components and the severity of pregnancy induced hypertension syndrome （ PIH ）, the unconcentrated urine samples from patients with PIH were analyzed on proteinuria components by electrophoresis. Methods Proteinuria components were analyzed by sodium dodecyl sulfate-agarose gel electrophoresis （SDS-AGE） in unconcentrated urine samples from PIH patients （PIH group, n = 114） and normal third trimester pregnant women （control group, n = 110）. Results Eleven kinds of urinary protein were detected in the PIH group and four in the control group. The results showed positive relationship between the urine protein component complexity and the severity of PIH （ P 〈 0. 01 ） . The relative percentage of IgG and transferrin showed significant positive correlation with the severity of PIH （P 〈 0. 01 ）. Conclusions Detecting proteinuria components by SDSAGE could help to distinguish pathological proteinuria induced by PIH from physiological proteinuria, which were also valuable to evaluate the severity of PIH.

关 键 词：尿蛋白 妊娠并发症 高血压 

分 类 号：R714.246[医药卫生—妇产科学] R733.3[医药卫生—临床医学]