丁香中丁香酚的高效毛细管电泳测定  

HPCE Determination of Eugenol in Eugenia caryophyllata Thumb.

作　　者：田益玲[1] 祝彦忠[1] 陈冠华[1] 齐小菊[1] 

机构地区：[1]河北农业大学食品科技学院,保定071001

出　　处：《药物分析杂志》2005年第11期1322-1324,共3页Chinese Journal of Pharmaceutical Analysis

基　　金：河北省自然科学基金(No.202281);河北省教育厅自然科学基金(No.2000257)资助项目

摘　　要：目的:探讨高效毛细管电泳法测定公丁香和母丁香中丁香酚的含量。方法:采用 Bio-Focus 3000型高效毛细管电泳仪,无涂层毛细管柱(50 cm×50 μm,有效长度为45.4 cm),检测波长205 nm,压力进样137.8 kPa·s,分离电压15 kV,温度25℃,运行缓冲液:0.06 mol·L^(-1)碳酸盐缓冲液(用1 mol·L^(-1)氢氧化钠溶液调 pH 至9.7)。进样前毛细管分别以水(30s)、1 mol·L^(-1)的盐酸(30 s)、水(30 s)、1 mol·L^(-1)氢氧化钠(30 s)、水(60 s)和运行缓冲液(120 s)的程序进行冲洗。结果:该法丁香酚的平均回收率为97.3%,RSD 为1.75。结论:该法简便快速,消耗少,灵敏度高,重复性好。Objective：A method for determination of eugenol in Flos Caryophylli and Fructus caryophylli by high performance capillary electrophoresis （ HPCE ） was described. Method： Bio - Focus 3000 apparatus equipped with UV detector untreated fused -silica capillary（50 cm × 50 μm,45.4 cm effective length）were used. The run buffer was 0. 06 mol · L^-1 carbonate （adjusted to pH = 9. 71 with 1 mol · L^-1 sodium hydroxide）. Before sample injection, the capillary was rinsed water（ for 30 s）, 1 mol · L^- 1 hydrochloric acid（ for 30 s）, water（ for 30 s）, 1 mol · L^-1 sodium hydroxide（for 30 s）and runing buffer for 120 s, respectivaly. 15 kV was applied for the separation. Pressure injection was 137.8 kPa · s, and the wavelength of detector was 205 nm. Results：The average recovery of eugenol was 97. 3% ,relative standard deviation was 1.7% ,respectively（ n =5 ）. Conclusion：The method is simple, rapid, low consumed and sensitive with repeatability.

关 键 词：高效毛细管电泳 丁香 丁香酚 

分 类 号：R284.1[医药卫生—中药学] R692.6[医药卫生—中医学]