HPLC法测定芜荽籽中的绿原酸  

Quantitative Determination of Chlorogenic Acid in Coriandurm Seed by HPLC

作　　者：李锋[1] 解成喜[1] 张丽静[1] 范维刚[1] 

机构地区：[1]新疆大学理化测试中心,乌鲁木齐830046

出　　处：《药物分析杂志》2005年第11期1391-1392,共2页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:采用 HPLC 法测定芫荽籽中绿原酸的含量。方法:色谱柱为 Hypersil-ODS 柱(6.0 mm×125 mm,5μm),以甲醇-0.4%乙酸(25:75)为流动相,流速为1.0 mL·min^(-1),柱温为室温,检测波长328 nm,进样量5 μL。结果:在上述色谱条件下,绿原酸对照品的峰面积与其质量在0.015～0.106μg范围内线性关系良好,回归方程为 Y=9903.85X+12.72,r=0.9996,平均回收率为98.3%(n=3)。结论:此方法简单快速,结果准确。Objective：An HPLC method for the quantitative determination of chlorogenic acid in Coriandurm seed was investigated. Method ：The sample was extracted with 50% methanol solution and then analyzed by HPLC. The operating conditions were Hypersil- ODS column（6. 0 mm× 125 mm,5μ m）at room temperature,mobile phase of methanol-0. 4% acetic acid（25： 75）at 1.0 mL · min^-1 and UV detection at 328 nm. Results：There was a good linear relationship between the area of chlorogenic acid and the mass of sample in the range of 0. 015 - 0. 106 μg for chlorogenic acid. The corresponding regression equation was Y = 9903. 85X ＋ 12. 72, r = 0. 9996. The average recovery was 98.3% （ n = 3 ）. Conclusion：This method is simple, specific and accurate.

关 键 词：芫荽籽 绿原酸 HPLC 

分 类 号：R286.0[医药卫生—中药学] R282.71[医药卫生—中医学]