氧化型低密度脂蛋白的制备  

The Preparation of Oxidized Low Density Lipoprotein(ox-LDL)

作　　者：王拥军[1] 温玫[1] 庞式琪[1] 

机构地区：[1]首都医科大学宣武医院神经内科实验室

出　　处：《首都医学院学报》1996年第1期32-35,共4页

摘　　要：在体外用Ｃｕ^（２＋）诱导法使ＬＤＬ氧化修饰，利用紫外法监测氧化过程中所形成的结合二烯键，发现整个反应分为３个时相，即延迟期、快速反应期和分解期。利用硫代巴比妥反应物测定，发现氧化后ＬＤＬ中每ｍｇ蛋白脂质过氧化物增加了１５倍。Low density lipoprotein(LDL)was oxidized with Cu￣(2+)in vitro.A lag phase was followed by oxidave modification after addition of CuCl_2, and about 15 h,the reaction reach a peak. LDL oxidation was continuously monitored spectrophotometrically at 234 nm to follow the formation of conjugated dienes.Three phases were found in oxidation curve:Lag phase, propagation phase and decomposition phase. By determinating thiobarbituric acid reaction substances(TBARS),we found the lipid peroxidation increased as much as 15 times in LDL.

关 键 词：低密度脂蛋白 氧化修饰 制备 

分 类 号：Q503[生物学—生物化学]