氧化苦参碱的镇痛作用及其机制  

Analgesic action of oxymatrine and its mechanism

作　　者：刘芬[1] 刘洁[2] 陈霞[1] 王秋静[2] 李凯[1] 

机构地区：[1]吉林大学基础医学院药理学教研室,吉林长春130021 [2]吉林大学基础医学院机能科学实验中心,吉林长春130021

出　　处：《吉林大学学报（医学版）》2005年第6期883-885,共3页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅资助课题(20030430)

摘　　要：目的:观察氧化苦参碱(oxymatrine,OMT)的镇痛作用,并初步探讨其镇痛作用机制。方法:将小鼠随机分成6组,即生理盐水组,OMT 50、100和200 mg.kg-1组,盐酸哌替啶注射液(10 mg.kg-1)组,L-硝基-精氨酸甲酯(L-NAME)20 mg.kg-1+OMT 100 mg.kg-1组。应用冰醋酸制备小鼠疼痛模型,以15 min内发生扭体次数为疼痛定量指标;将雌性小鼠随机分成6组,分组同前,将小鼠置于(55.0±0.5)℃的热板上制备疼痛模型,以小鼠舔后足反应的潜伏期为痛阈指标。结果:腹腔注射OMT 50、100和200 mg.kg-1能剂量依赖性地减少小鼠扭体反应次数,延长小鼠舔后足潜伏期,与生理盐水组比较,差异具有显著性(P<0.05,P<0.01,P<0.001);预先给予L-NAME能增强OMT对抗冰醋酸所致小鼠扭体反应的作用。结论:OMT能剂量依赖性地对抗冰醋酸及热板所致的小鼠疼痛反应,其镇痛作用可能不是完全通过NO-cGMP途径实现的。Objective To investigate the preliminary effects and mechanism of oxymatrine （OMT） on analgesia. Methods Mice were randomly divided into 6 groups; normal saline group, OMT groups with different doses （50, 100, and 200 mg·kg^-1）, pethidine hydrochloride （10mg·kg^-1） group, and NC-nitro-L-arginine methyl ester （L-NAME） 20 mg·kg^-1 ＋OMT100 mg·kg^-1 group. The number of writhing within 15 min was observed in pain mouse models caused by acetic acid. Female mice were randomly divided into 6 groups as the methods mentioned above. Mice were placed on the hot plate maintained at （55.0±0. 5）℃ and the latency of licking of the hind paws was observed. Results The number of writhing was decreased and the latency of licking of the hind paws was prolonged in a dose-dependent manner after intraperitoneal injection of OMT 50, 100, and 200mg·kg^-1 in mice （P〈0. 05, P〈0. 01, P〈0. 001）. L-NAME injected in advance enhanced the effect of OMT on antagonism of the writhing response caused by acetic acid. Conclusion These results suggest that OMT can antagonize the acute pain caused by acetic acid and hot plate in a dose-dependent manner in mice. The analgesic action of OMT may be not mediated completely by NO-cGMP pathway.

关 键 词：苦参碱/药理学 镇痛 L-硝基-精氨酸甲酯 疼痛测定 动物 实验 

分 类 号：R285.5[医药卫生—中药学]