自拟舒肝解郁灵对抑郁模型小鼠行为及血清中白细胞介素2、白细胞介素6和皮质醇的影响  

Effects of Shugan Jieyuling self-made on behavior and levels of serum IL-2,IL-6 and cortisol in depression models

作　　者：李秋波[1] 姜赛琳[2] 姚迪[1] 张萍[1] 李有田[1] 徐彩云[3] 许丹[1] 

机构地区：[1]吉林大学第一医院中医科,吉林长春130021 [2]吉林大学第一医院手术室,吉林长春130021 [3]吉林大学基础医学院动物实验室,吉林长春130021

出　　处：《吉林大学学报（医学版）》2005年第6期899-902,共4页Journal of Jilin University：Medicine Edition

基　　金：吉林省中药管理局资助课题(200234);吉林省科技厅资助课题(20030903)

摘　　要：目的:探讨自拟舒肝解郁灵对抑郁模型小鼠行为及血清中白细胞介素2(IL-2)、白细胞介素6(IL-6)和皮质醇的影响。方法:选择Open-field法评分相近的成年小鼠,应用分养和长期不可预知的中等强度应激造成小鼠抑郁模型,观察自拟舒肝解郁灵对该模型小鼠的作用,定期测定各组小鼠体重变化及观察小鼠行为的改变;利用放免法测定小鼠血清中IL-2、IL-6和皮质醇含量的变化。结果:给药前抑郁组小鼠体重增加数与对照组比较显著下降(P<0.01),精神状态及行为都有所改变,表现为易惊、恐惧和无助感,模型小鼠运动次数与对照组比较明显减少(P<0.05);同时抑郁模型小鼠血清中的IL-2、IL-6及皮质醇含量均明显高于对照组(P<0.05)。自拟舒肝解郁灵(大、小剂量组)和百忧解给药21 d后,小鼠体重降低有所改善,精神状态改善,血清中IL-2、IL-6及皮质醇含量降低,明显低于生理盐水组(P<0.01)。结论:IL-2、IL-6及皮质醇可作为诊断抑郁症的指标,自拟舒肝解郁灵对抑郁动物模型的各项指标和行为有明显改善作用。Objective To study the effects of Shugan Jieyuling self-made （SJSM） on behavior, levels of serum IL-2, IL-6 and eortisol in depression model mice. Methods The adult mice separately raised and treated with chronic unpredictable middle stress stimulus were used to establish depression models. The curative effect of SJSM was observed in depression model mice. The changes of weight and behavior were detected in a period. Radioimmunoassay （RIA） was used to examine the contents of serum IL-2, IL-6 and eortisol levels. Results The increased weights of the depression model mice were declined compared with the normal mice before administration. The mental state and behavior of the depression mice were changed. The mice were starling, dreadful, helpless and immobile. At the same time the contents of serum IL-2, IL-6 and eortisol were obviously lower than those of the normal mice. SJSM （large and low doses） and Baiyoujie changed the increased weights and behaviors of the depression after administration for 21 d. The mental state was meliorated simultaneously, and the serum IL-2, IL-6 and cortisol levels in the depression model mice were decreased significantly compared with normal mice. Conclusion The levels of serum IL-2, IL-6 and cortisol may be the guideline for the diagnosis of depression disease. SJSM can obviously improve both the symptoms of the depression models and the levels of serum IL-2, IL-6 and cortisol.

关 键 词：舒肝解郁灵 抗抑郁药 白细胞介素2 白细胞介素6 皮质醇 疾病模型 动物 

分 类 号：R285.5[医药卫生—中药学] R-332[医药卫生—中医学]