银新杨中与DRE元件结合的转录因子的克隆及鉴定分析  

Isolation and Characterization of a DRE-binding Transcription Factor from Yinxin Poplar (Populus alba×P. alba var. pyramidalis)

作　　者：秦红霞[1] 贾志平 张海超 刘敬梅 宋玉霞[3] 

机构地区：[1]宁夏大学生命科学学院,银川750021 [2]北京扬华生物科技有限公司,北京100101 [3]宁夏农业生物技术重点实验室,银川750002

出　　处：《生物工程学报》2005年第6期906-910,共5页Chinese Journal of Biotechnology

摘　　要：DREB类转录因子特异地与DRE元件结合,在植物感受非生物逆境(干旱、高盐和低温)胁迫时,激活一系列逆境应答基因的表达。我们选用银新杨(Populusalba×P.albavar.pyramidalis)为材料,通过PCR和同源EST搜索的方法克隆得到了一个类DREB的基因,命名为PaDREB2。酵母单杂交实验表明,该基因编码的蛋白能特异地与DRE元件结合并激活下游报告基因的表达。用RTPCR的方法研究了PaDREB2的表达模式,结果表明PaDREB2受低温、干旱和高盐的胁迫诱导。Dehydration-Responsive Element Binding （DREB） transcription factors, specifically binding with dehydration reponsive element （DRE）, activate a variety of stress-responsive genes in plants under abiotic stresses （dehydration, high salt and low temperature）. Using PCR and homologous EST search, we isolated a DREB-like gene from Yirlxin poplar （ Populus alba × P. alba var. pyramidalis ） named PaDREB2. Yeast One-hybrid experiment demonstrated that PaDREB2 protein could function as a DREB transcription factor activating target gene expression by specifically binding to DRE cis-element. To study the expression pattern of PaDREB2, RT-PCR was carried out. And the results showed that PaDREB2 is induced by low temperature, drought and high salt.

关 键 词：DREB转录因子 脱水应答元件(DRE) PaDREB2 

分 类 号：S792.11[农业科学—林木遗传育种]