利用基因芯片技术研究两品种鸡脂肪组织差异表达基因  

cDNA Microarray on Differentially Expressed Genes of Adipose Tissue in Two Breeds Chicken

作　　者：王洪宝[1] 王启贵[1] 李辉[1] 

机构地区：[1]东北农业大学动物科技学院,哈尔滨150030

出　　处：《生物工程学报》2005年第6期979-982,共4页Chinese Journal of Biotechnology

基　　金：国家高技术研究发展计划(863)项目(No.2002AA211021);教育部优秀青年教师资助计划项目(No.1985)。~~

摘　　要：应用包含9024条鸡cDNA的表达谱芯片,对从两品种鸡脂肪组织抽提及纯化的mRNA进行芯片杂交,并对基因表达谱进行分析,旨在筛选高脂肉鸡和白耳蛋鸡脂肪组织差异表达的基因,探讨造成两品种体脂性状差异的分子生物学机理。结果按差异显著阳性标准分析,共筛选出差异表达基因67条,主要涉及脂类代谢、能量代谢、细胞骨架构成、转录和剪接因子以及蛋白质合成与分解等相关基因,此外,还筛选出一些尚未在GenBank上登陆的序列,推测可能是未知的新基因,它们在鸡脂类代谢的过程所起到的作用还需进一步实验证明。cDNA microarray containing 9024 cDNAs was used to construct gene expression profile in order to screen differentially expressed genes of adipose tissue between broiler and Bai＇ er and investigate the molecular mechanism related with body fatness traits between the two breeds. Sixty seven differentially expressed genes, being involved in fat metabolism, energy metabolism, cytoskeleton, transcription and splicing factor, protein synthesis and degradation, were screened out. Furthermore, some genes that had no annotation in GenBank were screened out, they were presumed to be unknown new genes. The roles that they may play in chicken fat metabolism need clarify later.

关 键 词：鸡 基因芯片 脂肪 基因表达谱 

分 类 号：S831[农业科学—畜牧学] Q789[农业科学—畜牧兽医]