蔗糖和光对三裂叶野葛毛状根生长及次生物质产生的影响  

Effects of Sucrose and Light on the Growth and Production of Secondary Metabolites in Pueraria phaseoloides Hairy Roots

作　　者：何含杰[1] 梁朋[1] 施和平[1] 

机构地区：[1]华南师范大学生命科学学院广东省植物发育生物工程重点实验室,广州510631

出　　处：《生物工程学报》2005年第6期1003-1008,共6页Chinese Journal of Biotechnology

摘　　要：研究了蔗糖浓度和光对固体培养的三裂叶野葛毛状根生长及其总异黄酮和葛根素产生的影响。结果表明:在供试的分别添加1%、3%、5%、7%和9%蔗糖的MS固体培养基中,3%蔗糖能促进三裂叶野葛毛状根的生长及其异黄酮类化合物和葛根素的积累;培养20d后,其生物量达到0.48g(DW,干重)瓶,总异黄酮和葛根素含量分别为25.44mgg(DW)和11.64mgg(DW)。与添加3%蔗糖的MS培养基培养的三裂叶野葛毛状根相比,含5%蔗糖的培养基培养的毛状根干重增殖倍数提高了7.0%,而含1%、7%和9%蔗糖的培养基培养的毛状根干重增殖倍数分别下降62.4%、42.8%和65.3%;其总异黄酮含量分别降低57.4%、13%和33.4%,葛根素含量分别下降47.9%、15.8%和35.1%,但其毛状根培养物的可溶性糖含量则分别增加了0.52、1.45和1.54倍。暗培养30d的毛状根的生物量达到0.83g(DW)瓶,分别比蓝光和白光培养的毛状根提高37.1%和23.3%。在蓝光和白光下培养的部分毛状根的表面呈淡绿色;但白光处理的毛状根中总异黄酮含量比蓝光和暗培养处理的分别提高了14.7%和19.2%;蓝光抑制毛状根中葛根素含量的积累,白光和暗培养的毛状根培养物中的葛根素含量分别是蓝光处理的1.61倍和1.52倍。Effects of sucrose concentrations and light on the growth and production of total isoflavones and puerarin in Pueraria phaseoloides hairy roots cultured onto solid MS media supplemented with 1%, 3% , 5%, 7% and 9% sucrose, respectively, were investigated. The results showed that among the sucrose concentrations tested, 3% sucrose in the medium enhanced the growth and stimulated accumulation of total isoflavones and puerarin in P. phaseoloides hairy roots, After cultured for 20 days, the hiomass of hairy roots reached 0.48g（ DW dry weight ） /flask and its contents of total isoflavones and puerarin were 25.44mg/g（DW） and 11.64mg/g（DW）, respectively. In comparison with 3% sucrose, the dry weight proliferation of hairy roots cultured with 5% sucrose was increased by 7.0% , while cultured with 1% ,7% and 9% sucrose, the dry weight proliferation of hairy roots was decreased by 62.4%, 42.8% and 65.3%, their total isoflavones content was decreased by 57.4%, 13% and 33.4% and their puerarin content was decreased by 47.9% , 15.8% and 35.1% ; hut their content of total soluble sugars was increased 0.52,1.45 and 1.54 times, respectively. Compared with hairy roots in blue light and white light,the biomass of hairy roots cultured in the dark for 30 days was 0.83g（DW）/flask and was increased by 37.1% and 23.3 %, respectively. The content of total isoflavones in hairy roots cultured in white light was as much as 1.15 times and 1.19 times that in blue light and in the dark, respectively. It was also observed that hairy roots euhured in blue light and white light partly became light green and that blue light could inhibit accumulation of puerarin in hairy roots and the puerarin content in hairy root cultured in white light and in the dark were 1.61 times and 1.52 times that in blue light, respectively.

关 键 词：三裂叶野葛 毛状根 总异黄酮 葛根素 蔗糖 光 

分 类 号：S567.239[农业科学—中草药栽培]