人血清骨钙素ELISA测定法及临床应用  

Human Serum Osteocalcin Determined by ELISA and its Clinical Application

作　　者：褚为靖[1] 张海文[1] 薛延[1] 祝恕德[2] 董洁英[2] 李银水[2] 

机构地区：[1]北京创伤骨科研究所,100035 [2]北京积水潭医院

出　　处：《天津医药》1996年第5期270-272,共3页Tianjin Medical Journal

摘　　要：采用酶联免疫学方法(ELISA)测定人血清完整骨钙素(BGP)水平,观察全年龄组BGP的正常人参考值范围,提出其在某些疾病中的临床意义。研究对象选取正常人254例,内科疾患79例,妇科妊高征8例。结果表明:BGP水平随年龄和性别变化,5～15岁青少组的BGP水平出现一明显峰值。与同年龄正常对照组相比,肾功能不全患者、佝偻病患儿、多发性骨髓瘤患者和骨质疏松患者BGP水平均升高(P<0.001,P<0.05,P<0.02及P<0.05);妊高征患者则低于对照组(P<0.05)。本研究结论:BGP作为骨代谢研究的灵敏指标,对某些与骨代谢有关的内科疾病的诊断及其严重程度的判别有参考意义。Intact osteocalcin(BGP) levels in human serum were measured by enzyme linked immunosorbent assay(ELISA). Serum BGP levels in 254 normal subjects and 87 patients with various internal diseases or pregnancy-induced hypertension were determined. The results showed that BGP levels changed with age and sex,but a significant peak appeared in 5-10 year age groups. Compared with normal control groups,BGP levels were elevated in renal failure,rickets,multiple myeloma and osteoporosis(P <0. 001,P<0. 05,P<0. 02 and P<0. 05 respectively). BGP levels in pregnancy-induced hypertension were significantly lower than normal control (P<0. 05). This study concluded that BGP as a sensitive index of bone metabolism has an important significance for the dignosis of internal diseases related to bone metabolism and the estimation of their seriousness.

关 键 词：骨钙素 肾功能 骨质疏松 骨髓肿瘤 ELISA 

分 类 号：R692.5[医药卫生—泌尿科学] R681[医药卫生—外科学]