葛根粗提物及葛根素对乙醇所致海马细胞HSP70表达的影响  

Effect of pueraria crude extreact and puerarin on ethanol-induced expression of heat shock protein 70 in embryonic mouse hippocampal cultures

作　　者：韩萍[1] 吴德生[1] 李文杰[2] 余增丽[2] 王旗[2] 

机构地区：[1]四川大学华西公共卫生学院,成都郑州大学公共卫生学院610041 [2]郑州大学公共卫生学院

出　　处：《中华医学杂志》2005年第41期2930-2933,共4页National Medical Journal of China

摘　　要：目的研究葛根粗提物和葛根素对乙醇引起的胎鼠海马细胞氧化损伤是否具有相同的保护作用.方法海马细胞的培养取材于孕18d的ICR胎鼠,采用RT-PCR和Western 印迹方法观察不同剂量的葛根粗提物及葛根素对乙醇引起的胎鼠海马细胞HSP70 mRNA及蛋白质表达的变化.结果 15 mg/L葛根粗提物和10 mg/L葛根素均可部分拮抗50～350mmol/L不同浓度乙醇引起的胎鼠海马细胞HSP70表达量的升高.结论葛根粗提物和葛根素均可降低乙醇所致海马细胞HSP70表达量的升高,证实两者具有相同的抗氧化作用.Objective To study if the Pueraria crude extreact （CP） and standard preparation of pure puerarin （SP） possess the same neuroprotective effects on the expression of heat shock protein （HSP） 70 in the embryonic mouse hippocampal ceils. Methods The hippocampus of 18-days-old mouse embryo was taken out and suspension of single cells was cultured. Ethanol was added to cause HSP70 mRNA expression. Solvent, ethanol of different concentrations （50,200, and 300 mmol/L）, SP ＋ ethanol, and SP ＋ ethanol were added respectrively. Western blotting was used to detect the expression of the expression of HSP70 mRNA. Results Ethanol of different concentrations increased the expression of HSP70 mRNA and the protein in comparison with the solvent control group. SP and CP inhibited the expression of HSP70 mRNA and protein. Conclusion With identical effect of anti-oxydative stress, both SP and CP inhibit the increase of expression of HSP70 mRNA and protein, thus demonstrating Ⅰ vitro antioxidatice neuroprotection.

关 键 词：葛根素 乙醇 海马 热休克蛋白质类70 

分 类 号：R285[医药卫生—中药学]