砒石对哮喘小鼠血浆8-Isoprostane的影响  

Role of arsenolite on 8-isoprostane of asthmatic mice plasm

作　　者：黎东明[1] 谢佳星[1] 梁标[1] 

机构地区：[1]广东医学院呼吸疾病研究所,广东湛江524001

出　　处：《中国中药杂志》2005年第22期1758-1760,共3页China Journal of Chinese Materia Medica

摘　　要：目的:通过建立小鼠哮喘模型,给予4种剂量砒石治疗,检测血浆8-Isoprostane水平变化,探讨砒石对哮喘小鼠的治疗作用。方法:选用42只昆明种雄性小鼠,随机分为正常对照组和实验组,实验组建立哮喘模型,予地塞米松、砒石治疗,检测肺功能、血浆8-Isoprostane水平及BALF中WBC计数。结果:砒石治疗组小鼠t呼/t总和Pmax降低(P<0.05),哮喘组BALF中白细胞总数较正常对照组显著升高(P<0.001);经地塞米松和不同剂量砒石治疗后白细胞总数显著下降(P<0.001);哮喘组血浆中8-Isoprostane较正常对照组显著升高(P<0.001),经地塞米松和不同剂量砒石治疗后均显著下降;8-Isoprostane下降与白细胞计数呈正相关性。结论:哮喘小鼠机体存在氧化应激状态,砒石能够减轻其气道阻塞,降低气道高反应性,纠正氧化/抗氧化失衡。Objective： Through the estabhshment of mouse ovalbumin-sensitized asthmatic model and the observation of the 8-Isoprostane of plasm, to evaluate the therapeutic effects of arsenolite on asthmatic mice. Method： Forty-two healthy Kunming male mice were randomly divided into control group and experience groups, the latter were treated with dexamethasone, arsenolite. Lung function were tested, 8-isoprostane of plasm and WBC of BALF were measured. Result： Lung function improved after treating with dexamethasone or arsenolite. The WBC of asthmatic mice were significantly higher than those in control group, and decreased after treating with dexamethasone or arsenolite; 8-Isoprostane of plasm in asthmatic mice was higher than that of control group, and decreased after treating with dexamethasone or arsenolite. Conchusion： There is oxidant stress status in asthmatic mice. Arsenolite could lighten airway obstruction, reduce airway high response and redress oxidant stress status in asthmatic mice.

关 键 词：支气管哮喘 氧化应激 砒石 8-Isopmstane 

分 类 号：R285[医药卫生—中药学]