586例乙肝病毒携带产妇乳汁HBV-DNA的检测及分析  

Analysis HBV-DNA level in the milk in 586 postpartum women with hepatitis B

作　　者：周丽琴[1] 尤建飞[1] 吕时铭[1] 

机构地区：[1]浙江大学医学院附属妇产科医院检验科,杭州310006

出　　处：《浙江医学》2005年第11期816-817,821,共3页Zhejiang Medical Journal

摘　　要：目的了解乙型病毒性肝炎(简称乙肝)病毒携带产妇血清乙肝病毒DNA(HBV-DNA)含量、乙肝血清免疫学指标与乳汁中HBV-DNA含量及阳性率的关系,以指导母乳喂养。方法选择经血清学检测诊断为乙肝携带者的产妇586例(HBV-DNA定量分析呈阳性,免疫学指标提示为大三阳或小三阳),采用荧光定量聚合酶链反应(FQ-PCR)技术测定其乳汁中HBV-DNA含量,并对所有检测指标进行相关性分析。结果586例血清HBV-DNA阳性产妇的乳汁中检出HBV-DNA398例,总阳性率为67.9%,且乳汁HBV-DNA的阳性率随血清HBV-DNA含量的升高而增高;血清HBV-DNA的含量(3.58×108±1.40×108)与乳汁HBV-DNA的含量(5.30×106±2.6×106)呈正相关(r=0.569,P<0.05);大三阳产妇与小三阳产妇乳汁HBV-DNA阳性率差异有显著性意义(χ2=396.4,P<0.01)。结论血清HBV-DNA阳性产妇均应作乳汁HBV-DNA检测;乳汁HBV-DNA检测对正确指导乙肝产妇进行母乳喂养有一定实用价值。Objective To investigate HBV-DNA level in the milk in the postpartum women with Hepatitis B who are preparing for breast-feeding. Methods A total of 586 cases of postpartum women with Hepatitis B were selected to determine HBV-DNA level in their milk with fluorescent quantitative polymerase chain reaction （FQ-PCR）. Results Totally 586 cases of milks were detected HBV-DNA in all these HBV-DNA positive postpartum women, the positive rate was 67.9%. The HBV-DNA copy in the milk show positive relationship to the copy in the serum （r=0.569,P=0.002）. Serum immune indicatives also show significant correlation with the positive rate in the milk （P〈0.01）. Conclusions There are positive relationship in HBV-DNA level between serum and milk in postpartum women; HBV-DNA should be detected in the milk for HBV-DNA positive postpartum women who are preparing for breast-feeding.

关 键 词：乙型肝炎病毒 母婴传播 母乳喂养 荧光定量聚合酶链反应 

分 类 号：R446.5[医药卫生—诊断学]