柑橘黄龙病病原DNA微量提取方法比较  

Comparing of micro extraction methods of DNA from citrus huanglongbing pathogen

作　　者：邹敏[1] 宋震[1] 唐科志[1] 董鹏[2] 周常勇[1] 

机构地区：[1]中国农业科学院柑桔研究所,重庆400712 [2]西南农业大学植物保护学院

出　　处：《植物检疫》2005年第5期271-274,共4页Plant Quarantine

摘　　要：对比分析了3种黄龙病病原DNA微量提取方法,方法1和方法2分别采用Sandrine等人和Hung等人的黄龙病病原DNA提取方法,但取样量分别由100mg和250mg减少为20mg和10mg;方法3采用周常勇等人的微量核酸提取方法,取样量为10mg。实验结果表明方法1提取的病原DNA浓度低、杂质多,PCR效果差;方法2提取的病原DNA浓度较高、杂质少,PCR效果好,但提取周期较长,不适合大批量样品的PCR快速检测;方法3提取的病原DNA浓度较高、杂质少,PCR效果好,且提取速度快,适宜大批量样品的PCR快速检测。Three methods of micro extraction of DNA from citrus huanglongbing pathogen has been compared. Method 1 and method 2 are followed the methods conducted by Sandrine et al. and Hung et al., but the amount of the sample is reduced from 100mg and 250mg to 20mg and 10mg respectively. Method 3 is followed the method conducted by Zhou Changyong et al., the amount of the sample is 10mg. The result shows that the product of method 1 contains low concentration of DNA and much impurity, it is unfit for PCR detection of huanglongbing; product of method 2 contains high concentration of DNA and little impurity, it is fit for PCR detection of huanglongbing; product of method 3 contains high concentration of DNA and little impttrity, it is also fit for PCR detection of huanglongbing. Since the progress for extraction in method 3 is quicker than that of method 2, method 3 is more fit for PCR detection of a lot of samples.

关 键 词：柑橘 黄龙病 病原 DNA微量提取方法 PCR扩增 

分 类 号：S436.66[农业科学—农业昆虫与害虫防治]