雌激素对大鼠骨髓基质细胞增殖和分化的影响  

Effect of estrogen on proliferation and differentiation of rat bone marrow stromal cells

作　　者：王春先[1] 周磊[1] 周东风[1] 刘志强[1] 蔡海燕 

机构地区：[1]广东省口腔医院(南方医科大学附属口腔医院)种植中心,主治医师广东510280 [2]广东省荔湾区口腔医院,医师广东510140

出　　处：《口腔颌面修复学杂志》2005年第4期246-248,258,F0003,共5页Chinese Journal of Prosthodontics

基　　金：广东省医学科研基金资助项目(A2002119);广东省自然科学基金项目(32569)

摘　　要：目的:研究雌激素对大鼠骨髓基质细胞增殖和分化的影响,探讨其对成骨的影响。方法:体外培养4周龄雌性大鼠骨髓基质细胞,以不同浓度雌激素作用于成骨细胞,用噻唑蓝法测定细胞增殖情况;对硝基酚磷酸酯法测定细胞碱性磷酸酶活性;检测培养液中羟脯氨酸含量,了解细胞I型胶原的分泌情况;通过矿化结节计数反映细胞的矿化能力。结果:雌激素促进成骨细胞增殖,增加碱性磷酸酶活性;提高培养液中羟脯氨酸含量,峰值浓度为10-7mol/l,与对照组比较有统计学意义(P<0.05);雌激素显著提高细胞的矿化能力,10-7mol/l浓度作用最显著(+45.4%,P<0.05)。结论:雌激素促进成骨细胞增殖和分化,提高其矿化能力,从而刺激骨形成。Objective： To study the effect of estrogen on proliferation and differentiation of rat bone marrow stromal cells and to explore the mechanism of stimulation of bone formation by estrogen. Methods： Bone marrow stromal cells were isolated from four-week-old female Sprague-Dawley rats and cultured in DMEM medium to be induced into osteoblasts. They were cultured in medium with estrogen at different concentrations. The prolifereation, ALP activity and type-I pro-collagen of osteoblasts were examined by MTT, PNPP and assaying the level of hydroxyproline. The capacity of mineralization were measured by counting bone nodules. Results.. The cell quantity, the alkaline phosphatase activity and the level of hydroxyproline of osteoblasts were increased , and the number of nodules was significantly higher. The peak value of all these stimulating effects on osteoblasts was in 10^-7mol/L group. Conclusion.. Estrogen can increase proliferation, differentiation and the capability of mineralization of osteoblast and this may contribute to bone formation.

关 键 词：雌激素 骨髓基质细胞 增殖 分化 

分 类 号：R96[医药卫生—药理学]