1-(2-吡啶偶氮)-2-萘酚-磺酸与蛋白质结合反应的光谱法研究  

Study on the interaction between 1-(2-pyridylazo)-2-naphthol-sulfonic acid with cobalt(Ⅱ) complex and proteins

作　　者：胡瑞定[1] 李伟琦[2] 林秋月[1] 

机构地区：[1]浙江师范大学化学与生命科学学院 [2]金华广播电视大学,浙江金华321000

出　　处：《浙江师范大学学报（自然科学版）》2005年第4期407-412,共6页Journal of Zhejiang Normal University:Natural Sciences

基　　金：浙江省自然科学基金资助项目(Y404031)

摘　　要：采用荧光光谱法和吸收光谱法研究了1-(2-吡啶偶氮)-2-萘酚-磺酸(PAN-S)与牛血清白蛋白(BSA)的结合反应.研究表明,PAN-S对BSA的荧光猝灭机理为静态猝灭.测定了反应的结合常数(K=5.32×105～8.53×105L·mol-1)、荧光猝灭常数(Ksv=7.08×105L·mol-1)及结合位点数(n=2.88).进一步通过同步荧光法研究了PAN-S对BSA构象的影响,认为PAN-S主要结合在BSA的色氨酸残基附近.研究发现,PAN-S与BSA之间存在着类似于其同阳离子表面活性剂的静电作用.The binding reaction between 1-（2-pyridylazo）-2-naphthol-sulfonic acid （PAN-S） and bovine serum albumin（BSA） was studied by using fluorescence spectra and absorption spectra. The fluorescence spectra showed the quenching mechanism of BSA by PAN-S was a static quenching mechanism. The results showed that the binding constant K was 5.32×10^5～8.53×10^5L·mol^-1 , the quenching constant Ksv was 7.08×10^5L·mol^-1 , the number of binding sited n=2.88. The effect of PAN-S on conformation of BSA was further analyzed by using synchronous fluorescence spectrometry. By further research it was found that there were hydrophobic interactions between PAN-S and BSA. Meanwhile similar to cationic surfactand, static electric interactions existed between PAN-S and BSA.

关 键 词：牛血清白蛋白 1-(2-吡啶偶氮)-2-萘酚-磺酸 荧光光谱 吸收光谱 

分 类 号：O614.33[理学—无机化学]